Jun 21, 2023
Extreme DNA resolution: Spatially multiplexed single-molecule translocations through a nanopore at controlled speeds
Posted by Shailesh Prasad in categories: biotech/medical, genetics, nanotechnology
Aleksandra Radenovic, head of the Laboratory of Nanoscale Biology in the School of Engineering, has worked for years to improve nanopore technology, which involves passing a molecule like DNA through a tiny pore in a membrane to measure an ionic current. Scientists can determine DNA’s sequence of nucleotides—which encodes genetic information—by analyzing how each one perturbs this current as it passes through. The research has been published in Nature Nanotechnology.
Currently, the passage of molecules through a nanopore and the timing of their analysis are influenced by random physical forces, and the rapid movement of molecules makes achieving high analytical accuracy challenging. Radenovic has previously addressed these issues with optical tweezers and viscous liquids. Now, a collaboration with Georg Fantner and his team in the Laboratory for Bio-and Nano-Instrumentation at EPFL has yielded the advancement she’s been looking for—with results that could go far beyond DNA.