Lifeboat Foundation

One of the most difficult aspects of gene therapy might be ensuring that it gets into the right cells safely so it can have a therapeutic effect. Researchers have now created a new way to deliver various types of RNA cargo to cells, which utilizes one of the human body’s natural proteins to create particles that can bind to RNA. This approach, called SEND, may help reduce any immune response that would be mounted against a gene therapy. The work has been reported in Science.

Current delivery systems are not efficient, may integrate their cargo improperly, and can cause serious immune reactions. “The biomedical community has been developing powerful molecular therapeutics, but delivering them to cells in a precise and efficient way is challenging,” said senior study author Feng Zhang, Ph.D., a core institute member at the Broad Institute, among many other appointments. “SEND has the potential to overcome these challenges.”

Batteries are widely used in everyday applications like powering electric vehicles, electronic gadgets and are promising candidates for sustainable energy storage. However, as you’ve likely noticed with daily charging of batteries, their functionality drops off over time. Eventually, we need to replace these batteries, which is not only expensive but also depletes the rare earth elements used in making them.

A key factor in battery life reduction is the degradation of a battery’s structural integrity. To discourage structural degradation, a team of researchers from USC Viterbi School of Engineering are hoping to introduce “stretch” into battery materials so they can be cycled repeatedly without structural fatigue. This research was led by Ananya Renuka-Balakrishna, WiSE Gabilan Assistant Professor of Aerospace and Mechanical Engineering, and USC Viterbi Ph.D candidate, Delin Zhang, as well as Brown University researchers from Professor Brian Sheldon’s group. Their work was published in the Journal of Mechanics and Physics of Solids.

A typical battery works through a repetitive cycle of inserting and extracting Li-ions from electrodes, Zhang said. This insertion and extraction expands and compresses the electrode lattices. These volume shifts create microcracks, fractures and defects over time.

Top bioengineer sheds light on recent organ-on-a-chip developments, highlights challenges and predicts a multi-organ future.

New information from a study reported in Stem Cells might result in more effective treatments for osteoarthritis and other cartilage diseases, as well as hereditary disorders affecting cartilage development. Their findings might also point to a new way to accelerate stem cell differentiation for bioengineering cartilage, the researchers say.

What we’ll soon see is the ultimate self-directed evolution fueled forward by gene editing, genetic engineering, reproduction assisted technology, neuro-engineering, mind uploading and creation of artificial life. Our success as a technological species essentially created what might be called our species-specific “success formula.” We devised tools and instruments, created new methodologies and processes, and readjusted ecological niches to suit our needs. And our technology shaped us back by shaping our minds. In a very real sense, we have co-evolved with our technology. As an animal species among many other species competing for survival, this was our unique passage to success.

#TECHNOCULTURE : #TheRiseofMan #CyberneticTheoryofMind

Continue reading “The Rise of Man: What Was Our Ultimate Success Formula As a Species?” »

Craig Montell is a professor at the University of California, Santa Barbara, who helped lead the research. He said in a statement that by removing the two eye receptors, the team was able to “eliminate CO2-induced target recognition without causing blindness.”

Female Aedes aegypti search out blood meals in humans to develop eggs. They use several different senses to find those meals. One of the main identifying tools is the smell of carbon dioxide (CO₂). When a human breathes out CO₂, the mosquitoes become more active and begin looking for targets to bite.

The research team said this search generally begins with the mosquito flying toward the direction of the released CO₂. When seeking out targets, the insects search for dark objects. Once the mosquitoes are within close range, they can also sense heat from skin and additional skin smells to help guide them to a human.

Continue reading “Gene Editing Used to Block Mosquitos’ Ability to Identify Targets” »

I am pleased to announce that my lead-author review paper has been published in ACS Nano! If you are interested in learning about the convergence of synthetic biology and adenoviral gene therapy, I encourage you to check out my paper.

If you cannot access the full text, I have also posted a local copy at the following link: https://logancollinsblog.files.wordpress.com/2021/08/synthet…s-2021.pdf.

#ACS #ACSNano #SyntheticBiology #GeneTherapy #Biology #Biotech #Science #Biotechnology #Nanotechnology #Adenovirus #Engineering #Virology

Continue reading “Synthetic Biology Approaches for Engineering Next-Generation Adenoviral Gene Therapies” »

Many drugs show promising results in laboratory research but eventually fail clinical trials. We hypothesize that one main reason for this translational gap is that current cancer models are inadequate. Most models lack the tumor-stroma interactions, which are essential for proper representation of cancer complexed biology. Therefore, we recapitulated the tumor heterogenic microenvironment by creating fibrin glioblastoma bioink consisting of patient-derived glioblastoma cells, astrocytes, and microglia. In addition, perfusable blood vessels were created using a sacrificial bioink coated with brain pericytes and endothelial cells. We observed similar growth curves, drug response, and genetic signature of glioblastoma cells grown in our 3D-bioink platform and in orthotopic cancer mouse models as opposed to 2D culture on rigid plastic plates. Our 3D-bioprinted model could be the basis for potentially replacing cell cultures and animal models as a powerful platform for rapid, reproducible, and robust target discovery; personalized therapy screening; and drug development.

Cancer is the second leading cause of death globally. It is estimated that around 30 to 40% of patients with cancer are being treated with ineffective drugs ; therefore, preclinical drug screening platforms attempt to overcome this challenge. Several approaches, such as whole-exome or RNA sequencing (RNA-seq), aim to identify druggable, known mutations or overexpressed genes that may be exploited as a therapeutic target for personalized therapy. More advanced approaches offer to assess the efficacy of a drug or combinations of drugs in patient-derived tumor xenograft models or in vitro three-dimensional (3D) organoids. Unfortunately, most of the existing methods face unmet challenges, which limit their efficacy. For instance, cells can become quiescent or acquire somatic mutations while growing many generations on plastic under the influence of static mechanical forces and in the absence of functional vasculature.

Quick vid and a reminder of the 4th conference of Lifespan.io is this weekend.

Gene editing can make stem cells invisible to the immune system, making it possible to carry out cell therapy transplants without suppressing the patients’ immune response. Scientists in the US and Germany used immune engineering to develop universal cell products that could be used in all transplant patients. The idea is to create stem cells that evade the immune system; these hypoimmune stem cells are then used to generate cells of the desired type that can be transplanted into any patient without the need for immunosuppression, since the cells won’t elicit an immune response. They used CRISPR-Cas9 to knock out two genes involved in the major histocompatibility complex, which is used for self/non-self discrimination. They also increased the expression of a protein that acts as a “don’t eat me” signal to protect cells from macrophages. Together, these changes made the stem cells look less foreign and avoid clearance by macrophages. The team then differentiated endothelial cells and cardiomyocytes from the engineered stem cells, and they used these to treat three different diseases in mice. Cell therapy treatments using the hypoimmune cells were effective in rescuing hindlimbs from vascular blockage, preventing lung damage in an engineered mouse model, and maintaining heart function following a myocardial infarction. Immunosuppression poses obvious risks to a patient, and generating custom cells for transplant therapy is often prohibitively expensive. The development of universal donor cells that can be used as therapeutics could bring the cost down significantly, making cellular therapeutics available to many more patients in a much safer way.

Continue reading “CRISPR Development Makes Stem Cells ‘Invisible’ to Immune System Without Immunosuppressants” »