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Category: health – Page 249

Genomic Editing of Non-Coding RNA Genes with CRISPR/Cas9 Ushers in a Potential Novel Approach to Study and Treat Schizophrenia

Circa 2017

Schizophrenia is a genetically related mental illness, in which the majority of genetic alterations occur in the non-coding regions of the human genome. In the past decade, a growing number of regulatory non-coding RNAs (ncRNAs) including microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) have been identified to be strongly associated with schizophrenia. However, the studies of these ncRNAs in the pathophysiology of schizophrenia and the reverting of their genetic defects in restoration of the normal phenotype have been hampered by insufficient technology to manipulate these ncRNA genes effectively as well as a lack of appropriate animal models. Most recently, a revolutionary gene editing technology known as Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated nuclease 9 (Cas9; CRISPR/Cas9) has been developed that enable researchers to overcome these challenges. In this review article, we mainly focus on the schizophrenia-related ncRNAs and the use of CRISPR/Cas9-mediated editing on the non-coding regions of the genomic DNA in proving causal relationship between the genetic defects and the pathophysiology of schizophrenia. We subsequently discuss the potential of translating this advanced technology into a clinical therapy for schizophrenia, although the CRISPR/Cas9 technology is currently still in its infancy and immature to put into use in the treatment of diseases. Furthermore, we suggest strategies to accelerate the pace from the bench to the bedside. This review describes the application of the powerful and feasible CRISPR/Cas9 technology to manipulate schizophrenia-associated ncRNA genes. This technology could help researchers tackle this complex health problem and perhaps other genetically related mental disorders due to the overlapping genetic alterations of schizophrenia with other mental illnesses.

Keywords: CRISPR/Cas9; gene editing; lncRNAs; miRNAs; non-coding RNAs; schizophrenia.

Nature-inspired self-sensing materials could lead to new developments in engineering

The cellular forms of natural materials are the inspiration behind a new lightweight, 3D printed smart architected material developed by an international team of engineers.

The team, led by engineers from the University of Glasgow, mixed a common form of industrial plastic with carbon nanotubes to create a material which is tougher, stronger and smarter than comparable conventional materials.

The nanotubes also allow the otherwise nonconductive plastic to carry an throughout its structure. When the structure is subjected to mechanical loads, its electrical resistance changes. This phenomenon, known as piezoresitivity, gives the material the ability to “sense” its structural health.

Design of effective self-powered SnS2/halide perovskite photo-detection system based on triboelectric nanogenerator

On account of the improvement the Internet of things (IoTs) and smart devices, our lives have been noticeably facilitated in the past few years. Machines and devices are becoming more ingenious with the help of artificial intelligence and various sensors1,2. So, integrated circuits are necessary to provide convenient and effectual communication3 Since the first report on TENG by Wang’s group in 20124, triboelectric systems have been recognized as a proper choice to harvest and convert the energy from the environment5,6. Photodetectors, as one of the most significant types of sensors that can precisely convert incident light into electrical signals have attracted increasing attention in recent years. Various applications including photo-sensors, spectral analysis7,8, environment monitoring9, communication devices10, imaging11, take advantage of narrow band or broad band photodetectors from ultraviolet to terahertz wavelenght. Literature reviews show that the heterojunction/heterostructure based on 2D/3D materials have been widely used in PD applications. In fact, to attain high performance of PDs based heterojunction, the built-in electrical field is needed to suppress the photogenerated recombination and stimulating collection12. Although, Si based PDs offer reliably high performance results, their complexity and expensive manufacturing process have limited their expansion and adoptability for industrial purposes13,14,15. Hence, most available PDs are designed based on external power supplies such as electrochemical batteries for signal production and processing, their design not only increases the sensor’s dimension and weight, but also creates limitations for sensor maintenances16 which is not proper in the IoTs. In 2014, ZH Lin et al. and Zheng et al. represented an investigation on the self-powered PD based on TENG system3,17, and since then, self-driven PDs have been extensively investigated2,5,9,18,19,20. These devices can find potential applications in health monitoring systems such as heart checking21 and health protection from some detrimental radiation such as high levels of UV radiance22.

But in the other hand, even though TENGs could be promise for using in wearable electronics, they still inevitably have limitations in power generation, sensing range, sensitivity, and also the sensing domain for the intrinsic limitations of electrification23,24,25. Moreover, due to high voltage, low current, and alternating current output of the TENGs, they cannot be used in order to supply power to electronic devices effectively without using power management circuits (PMCs) based on the LC modules. There are several reports that describe the importance of the impedance matching of the TENG and PMC units for better energy storage efficiency of the pulsed-TENG26,27. Without using the PMC unit, there are some challenges as a result of synching the TENG, as the power supply, and the consumption element such as the PD device. These challenges include the process of matching the resistance of the device and the impedance of the TENG to achieve effective performance of the self-powered system6,28.

In this study an efficient battery-free photodetector based on bulk heterojunction SnS2 nanosheets and perovskite materials has been designed and powered employing three different TENGs (GO paper/ Kapton, FTO/Kapton and hand/ FTO). In the first step for circuit designing to have better performance of the photodetector in coupling with TENG, the effect load resistance amount in the circuit on the impedance matching the TENG and the inner resistance of the photodetector, has been investigated through output current amplitude. The investigation, shows that to achieve the high amount of the photocurrent, the load resistance should be positioned in both critical zone of the out-put voltage of the TENG and the resistance range of high power density production of the TENG. In the second step, for investigation the effect of the dark resistance of the photodetector on out-put current of the self-powered photodetector, a device with very lower initial resistance (All-oxide Cu2O/ZnO photodetector) has been used with and without different load resistance in the circuit; in this regard, it is concluding that the initial resistance is too important to have proper design impedance matching circuit.

Dr Jerome H. Kim, MD, Director General, IVI — Safe, Effective, Affordable Vaccines For Public Health

Discovery, Development & Delivery Of Safe, Effective & Affordable Vaccines For Global Public Health — Dr. Jerome H. Kim, M.D., Director General, International Vaccine Institute (IVI)

Dr. Jerome H. Kim, M.D., is the Director General of the International Vaccine Institute (IVI — https://www.ivi.int/), a nonprofit International Organization established in 1997 as an initiative of the United Nations Development Programme (UNDP), dedicated to the discovery, development and delivery of safe, effective and affordable vaccines for global public health.

IVI is headquartered in Seoul and hosted by the Republic of Korea with 36 member countries and the WHO on its treaty.

Dr. Kim served as the Principal Deputy and Chief of the Laboratory of Molecular Virology and Pathogenesis at Military HIV Research Program at the Walter Reed Army Institute of Research (WRAIR), in addition to being the Project Manager for the HIV Vaccines and Advanced Concepts Evaluation Project Management Offices, U.S. Army Medical Material Development Activity.

Dr. Kim was also a Professor within the Division of Infectious Diseases of the Department of Medicine at the Uniformed Services University of the Health Sciences. From 2004 – 2009 he led the Army’s Phase III HIV vaccine trial (RV144), the first demonstration that an HIV vaccine could protect against infection, as well as subsequent studies that identified laboratory correlates of protection and sequence changes in breakthrough HIV infections after vaccination.

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Telehealth aims to crack open Paxlovid’s prescription bottleneck

After months of shortages, pharmacies across the United States are being stocked with drugs to treat Covid-19. Now, the bottleneck has shifted to getting a prescription — and patients and public health agencies are looking to telehealth for help.

“Sometimes we hear telehealth is going to cure all of our challenges with access to health care, and that’s just not the case,” said Michelle Morse, chief medical officer of New York City’s department of health. “It’s a significant step forward in access, and yet there are still equity concerns with telehealth. It’s not a panacea.”

The patients most likely to know about and navigate digital health platforms to access the antivirals are typically younger, more affluent, and already well-served by the health system. And while a small group of public health departments are offering free telehealth, the end of the Health Resources and Services Administration Covid-19 Uninsured program means that uninsured patients may struggle to pay for teleservices, let alone any Covid care.

Michelle Lin, an emergency physician who works on an urgent care telehealth line in New York City, said many of the patients she sees turn to telehealth for Covid care have primary care doctors, but have struggled to get an appointment with them on short notice.

Researchers testing light technology that could protect against the next pandemic

New technology could add another layer of protection against the next pandemic by simply turning on a light. Researchers are exploring a new way of using ultraviolet light to make indoor air safer.

“It’s been known for 80 years or so that ultraviolet light can kill bacteria and inactivate viruses in the air so that they’re no longer infectious,” Don K. Milton, professor of occupational and environmental health at the University of Maryland School of Public Health, told CBS News.

Conventional UV-C light has been used extensively in places like hospitals, homeless shelters and prisons. But that conventional UV light can damage the skin and eye, so should not be shined directly at people.

Researchers find new function performed by almost half of brain cells

* Astrocytes play a variety of roles with neurons, but until now, scientists did not know that these cells carry electrical impulses.

* Applying new technology, Tufts University scientists recently discovered in mice that astrocytes are electrically active like neurons. Astrocytes play a variety of roles with neurons, but until now, scientists did not know that these cells carry electrical impulses.

Neurotransmitters are chemical messengers that facilitate the transfer of electrical signals between neurons and support the blood-brain barrier. Scientists have long understood that astrocytes control these substances to support neuronal health.

This study breaks ground in showing that neurons release potassium ions, which change the astrocytes’ electrical activity. This modulation affects how the astrocytes control neurotransmitters.

Until now, scientists could not image potassium activity in the brain.

Retinal Cell Map Could Advance Precise Therapies for Blinding Diseases

Researchers have identified distinct differences among the cells comprising a tissue in the retina that is vital to human visual perception. The scientists from the National Eye Institute (NEI) discovered five subpopulations of retinal pigment epithelium (RPE)—a layer of tissue that nourishes and supports the retina’s light-sensing photoreceptors. Using artificial intelligence, the researchers analyzed images of RPE at single-cell resolution to create a reference map that locates each subpopulation within the eye. A report on the research published in Proceedings of the National Academy of Sciences.

“These results provide a first-of-its-kind framework for understanding different RPE cell subpopulations and their vulnerability to retinal diseases, and for developing targeted therapies to treat them,” said Michael F. Chiang, M.D., director of the NEI, part of the National Institutes of Health.

“The findings will help us develop more precise cell and gene therapies for specific degenerative eye diseases,” said the study’s lead investigator, Kapil Bharti, Ph.D., who directs the NEI Ocular and Stem Cell Translational Research Section.

As New Omicron Subvariant Spreads, Here Are the Symptoms to Watch For

The BA.2 omicron subvariant still remains the dominant COVID strain across the U.S., but another subvariant has gained momentum in recent days.

BA.2.12.1, which health officials say appears to be up to 27% more contagious than BA.2, accounts for approximately 36.5% of cases nationwide, according to the most recent CDC weekly numbers.

While BA.2 accounts for approximately 75% of all cases in the country, it is said to make up at least 70% of the cases in the healthcare region encompassing New York, New Jersey, Puerto Rico and the Virgin Islands.

The Surprisingly Sophisticated Mind Of An Insect

Quinn SenaAuthor.

Tenor.

Jose Ruben Rodriguez Fuentes shared a link.

Carrie Arnold is a freelance health and science writer living in Virginia.

At the age of 20, I committed my first mass murder. I didn’t, of course, mean to kill anyone. But my good intentions meant nothing to the small mound of deceased fruit flies in the bottom of the vial.

My goal was simply to anesthetize them and then search their wrinkled, vellum wings and bulging eyes for mutations. It was a classic introductory genetics experiment, one taught to countless aspiring biologists for a century. I doused a cotton ball with ether, the fruity-smelling liquid that would render the flies temporarily unconscious (and easier to count). The instructor warned us to make sure the flies were completely knocked out, so that they didn’t wake up mid-experiment. So I left the ether-soaked cotton on the vial an extra minute or two. Just to be safe.

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