Lifeboat Foundation

Circa 2008 face_with_colon_three

Adult bone marrow (BM) houses a tiny pool of hematopoietic stem cells (HSCs) that have the ability to maintain not only themselves but also all the rest of highly turning over blood lineages throughout the mammalian life (1, 2). Hence, the ability to sustain HSC in tissue culture would allow serial introduction of gain or loss of function mutations efficiently in hematopoietic system. However, our failure to expand HSC in culture has hampered the use of this approach. In fact, BM suspension cultures lose rapidly their HSC content despite vigorous growth of progenitors and more differentiated cells at least for 3 weeks even in optimal cytokine milieu (3, 4). Therefore, the phenomenon of stem cell exhaustion or senescence may set the limits that make it impossible even in principle to expand HSC in culture for longer periods (5–7).

Mouse HSC do expand in vivo (8, 9), at least up to 8000-fold, as shown by Iscove and Nawa (9) through serial transplantation experiments that assessed carefully the input and output contents of HSC in each transfer generation. Recently also in vitro approaches have been improved and refined culture conditions with new growth factors can now support up to 30-fold expansion of mouse HSC ex vivo (10). However, since it is not clear to what extent external culture conditions can be improved, alternative but not mutually exclusive efforts to change the intrinsic properties of HSC have been taken. Seminal experiments in this respect by Humphries, Savageau and their colleagues have shown that ectopic expression of HOXB4 transcription factor in BM cells support the survival and expansion of HSC in vivo and importantly also in vitro (11–13). By rigorously monitoring the HSC content in their cultures of HOXB4-transduced BM cells, they found that HSC could be expanded up to 41-fold in the 2-week liquid cultures (13). HOXB4 belongs to a large family of HOX transcription factors that are crucial for the basic developmental processes in addition to their role in maintenance of different stem cell compartments.

Continue reading “Manipulation of immune system via immortal bone marrow stem cells” »

Chardan hosted its 4th Annual Chardan Genetic Medicines Conference in October 2020, featuring over 80 public and private companies representing in vivo gene therapy, ex vivo gene therapy, gene editing, RNA medicines, and other subsegments of the genetic medicines space. Among our various panels with preeminent thought leaders, we spoke with newly-minted Nobel laureate, President of the Innovative Genomics Institute, and Professor of Molecular and Cell Biology and Chemistry at UC Berkeley, Jennifer Doudna.

PhD about open questions and areas of innovation in the CRISPR gene editing space.

Circa 2014 face_with_colon_three

Continue reading “Scientists Swap Out Bad Memories For Good Ones In Mice” »

With billions of dollars flooding into longevity, what role will epigenetic clocks play in measuring and intervening in aging?

When Horvath first described epigenetic clocks, scientists began to speculate that altering them could reverse aging. After all, if certain patterns of DNA methylation at certain sites in cells in certain tissues of your body are hallmarks of aging, could shifting them somehow reverse aging?

More than six months after CAR-T cell treatment, five patients are in remission and have functional immune systems.

Optimizing Human-System Performance — Dr. Greg Lieberman, Ph.D., Neuroscientist / Lead, U.S. Army Combat Capabilities Development Command Army Research Laboratory, U.S. Army Futures Command

Dr. Greg Lieberman, Ph.D. (https://www.arl.army.mil/arl25/meet-arl.php?gregory_lieberman) is a Neuroscientist, and Lead, Optimizing Human-System Performance, at the U.S. Army Combat Capabilities Development Command, Army Research Laboratory (DEVCOM ARL).

Continue reading “Dr. Greg Lieberman, Ph.D. — Neuroscientist — Optimizing Human-System Performance, Army Research Lab” »

In nature, evolutionary chromosomal changes may take a million years, but scientists have recently reported a novel technique for programmable chromosome fusion that has successfully created mice with genetic changes that occur on a million-year evolutionary scale in the laboratory. The findings might shed light on how chromosomal rearrangements – the neat bundles of structured genes provided in equal numbers by each parent, which align and trade or mix characteristics to produce offspring – impact evolution.

In a study published in the journal Science, the researchers show that chromosome level engineering is possible in mammals. They successfully created a laboratory house mouse with a novel and sustainable karyotype, offering crucial insight into how chromosome rearrangements may influence evolution.

“The laboratory house mouse has maintained a standard 40-chromosome karyotype — or the full picture of an organism’s chromosomes — after more than 100 years of artificial breeding,” said co-first author Li Zhikun, researcher in the Chinese Academy of Sciences (CAS) Institute of Zoology and the State Key Laboratory of Stem Cell and Reproductive Biology. “Over longer time scales, however, karyotype changes caused by chromosome rearrangements are common. Rodents have 3.2 to 3.5 rearrangements per million years, whereas primates have 1.6.”

Biotech start-up Pretzel Therapeutics launched Monday with $72.5 million in Series A financing to develop novel, mitochondria-based therapies for rare genetic disorders and diseases of aging.

Pretzel plans to target mitochondrial diseases, a highly heterogenous group of conditions caused by DNA mutations in the mitochondria or the nucleus. These disorders are very rare, afflicting around one in 5,000 people.

Pretzel CEO Jay Parrish told BioSpace the funding “should enable us to get close to the clinic if not into the clinic with one or more programs.”

You won’t be able to blame it on your genetics anymore: with CRISPR, it’s so easy to hacn into your DNA. CRISPR technology is our future, and experiments with DNA hacking are booming. CRISPR biotechnology is not science fiction anymore, it is our very near future. Would you hack and reprogram your own DNA with CRISPR? Breaking the code of life, hacking DNA at home.

Welcome to the world of a new nature. We can now literally cut and paste DNA with the new CRISPR technology. There is a revolutionary development going on that will have major consequences for humans, plants and animals. The new biotechnology is here.

Continue reading “Hack your DNA with CRISPR — VPRO documentary” »