Named Suspended Layer Additive Manufacturing (SLAM), the approach enables the printing of a novel biomaterial that accurately simulates the structure of mammalian skin.
In fact, according to the researchers, the biomaterial is the first of its kind to simulate all three of the major layers found in skin – the hypodermis, the dermis, and the epidermis – making it a unique tri-layered skin equivalent. Early experiments suggest that the 3D bioprinted skin can be placed at the site of a wound to induce healing, reducing scar tissue in the process.
About the Bioprint FirstAid Handheld Bioprinter capabilities.
Astronauts on the International Space Station (ISS) are testing 3D bioprinted bandages made of their own cells that could be used to better heal flesh wounds in space.
The German Space Agency (DLR) is leading the experiment which was launched to the ISS at the end of December 2021 on SpaceX’s 24th commercial resupply mission. The payload contained the BioPrint FirstAid Handheld Bioprinter, which is designed to hold cells from astronauts within a bioink that can be used to apply bandages to wounds when needed.
While the experiment offers a promising tool for wound healing in space environments, it could also provide significant benefits back on earth, too.
Bio-Printing Complex Human Tissues & Organs — Dr. Anthony Atala, MD — Director, Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, Wake Forest University.
Dr. Anthony Atala, MD, (https://school.wakehealth.edu/Faculty/A/Anthony-Atala) is the G. Link Professor and Director of the Wake Forest Institute for Regenerative Medicine, and the W. Boyce Professor and Chair of Urology.
A practicing surgeon and a researcher in the area of regenerative medicine, fifteen applications of technologies developed Dr. Atala’s laboratory have been used clinically. He is Editor of 25 books and 3 journals, has published over 800 journal articles, and has received over 250 national and international patents. Dr. Atala was elected to the Institute of Medicine of the National Academies of Sciences, to the National Academy of Inventors as a Charter Fellow, and to the American Institute for Medical and Biological Engineering.
A major obstacle to widespread study and clinical use of 3D tissues is their short shelf-life, which may be anywhere from a just few hours to a few days. As in the case of an organ transplant, a bioprinted tissue must be transported rapidly to the location where it is needed, or it will not be viable. In the journal Matter on December 21st, researchers at Brigham and Women’s Hospital and Harvard Medical School describe their work combining 3D bioprinting with cryopreservative techniques to create tissues which can be preserved in a freezer at-196°C and thawed within minutes for immediate use.
“For conventional bioprinting, there is basically no shelf life. It’s really just print, and then use, in most cases,” says lead author Y. Shrike Zhang (@shrikezhang), a biomedical engineer at Brigham and Women’s Hospital. “With cryobioprinting, you can print and store in the frozen state for basically as long as you want.”
The use of 3D bioprinting to create artificial human tissue first appeared twenty years ago. As in conventional 3D printing, an ink is extruded layer by layer through a nozzle into a pre-specified shape. In the case of bioprinting, the ink is typically made up of a gelatin-like scaffolding embedded with living cells. Cryobioprinting works the same way, except the printing is performed directly onto a cold plate held at temperatures down to-20°C. After the tissues are printed, they are immediately moved to cryogenic conditions for long-term storage.
The 24thSpaceX cargo resupply services mission, targeted to launch in late December from NASA’s Kennedy Space Center in Florida, carries scientific research and technology demonstrations to the International Space Station. The experiments aboard include studies of bioprinting, crystallization of monoclonal antibodies, changes in immune function, plant gene expression changes, laundering clothes in space, processing alloys, and student citizen science projects.
A team of researchers from Texas A&M University’s Department of Biomedical Engineering has designed and 3D bioprinted a highly realistic model of a blood vessel.
The model is made of a newly nanoengineered, purpose-built hydrogel bioink and closely mimics the natural vascular function of a real blood vessel, as well as its disease response. The team hopes its work can pave the way for advanced cardiovascular drug development, expediting treatment approval while eliminating the need for animal and human testing altogether.
“A remarkably unique characteristic of this nanoengineered bioink is that regardless of cell density, it demonstrates a high printability and ability to protect encapsulated cells against high shear forces in the bioprinting process,” said Akhilesh Gaharwar, associate professor at the university and co-author of the study. “Remarkably, 3D bioprinted cells maintain a healthy phenotype and remain viable for nearly one month post-fabrication.”
The world of lab-grown meats is fast filling with all kinds of tasty bites, from burgers, to chicken breasts, to a series of increasingly complex cuts of steak. Expanding the scope of cultured beef are scientists from Japan’s Osaka University, who have leveraged cutting-edge bioprinting techniques to produce the first lab-grown “beef” that resembles the marbled texture of the country’s famed Wagyu cows.
From humble beginnings that resembled soggy pork back in 2,009 to the classic steaks and rib-eyes we’ve seen pop up in the last few years, lab-grown meat has come along in leaps and bounds. The most sophisticated examples use bioprinting to “print” living cells, which are nurtured to grow and differentiate into different cell types, ultimately building up into the tissues of the desired animal.
The Osaka University team used two types of stem cells harvested from Wagyu cows as their starting point, bovine satellite cells and adipose-derived stem cells. These cells were incubated and coaxed into becoming the different cell types needed to form individual fibers for muscle, fat and blood vessels. These were then arranged into a 3D stack to resemble the high intramuscular fat content of Wagyu, better known as marbling, or sashi in Japan.
Scientists from the University at Buffalo have developed a rapid new 3D bioprinting method that could represent a significant step towards fully-printed human organs.
Using a novel vat-SLA-based approach, the team have been able to reduce the time it takes to create cell-laden hydrogel structures, from over 6 hours to just 19 minutes. The expedited biofabrication method also enables the production of embedded blood vessel networks, potentially making it a significant step towards the lifesaving 3D printed organs needed by those on transplant waiting lists.
“Our method allows for the rapid printing of centimeter-sized hydrogel models,” explained the study’s lead co-author, Chi Zhou. “It significantly reduces part deformation and cellular injuries caused by the prolonged exposure to the environmental stresses you commonly see in conventional 3D printing.”
Biofabrication technologies, including stereolithography and extrusion-based printing, are revolutionizing the creation of complex engineered tissues. The current paradigm in bioprinting relies on the additive layer-by-layer deposition and assembly of repetitive building blocks, typically cell-laden hydrogel fibers or voxels, single cells, or cellular aggregates. The scalability of these additive manufacturing technologies is limited by their printing velocity, as lengthy biofabrication processes impair cell functionality. Overcoming such limitations, the volumetric bioprinting of clinically relevant sized, anatomically shaped constructs, in a time frame ranging from seconds to tens of seconds is described. An optical-tomography-inspired printing approach, based on visible light projection, is developed to generate cell-laden tissue constructs with high viability (85%) from gelatin-based photoresponsive hydrogels. Free-form architectures, difficult to reproduce with conventional printing, are obtained, including anatomically correct trabecular bone models with embedded angiogenic sprouts and meniscal grafts. The latter undergoes maturation in vitro as the bioprinted chondroprogenitor cells synthesize neo-fibrocartilage matrix. Moreover, free-floating structures are generated, as demonstrated by printing functional hydrogel-based ball-and-cage fluidic valves. Volumetric bioprinting permits the creation of geometrically complex, centimeter-scale constructs at an unprecedented printing velocity, opening new avenues for upscaling the production of hydrogel-based constructs and for their application in tissue engineering, regenerative medicine, and soft robotics.
