Something to post to your websites and to vote online.
Aubrey de Grey can get $1.5 million for the Methuselah Foundation if enough people vote.
Voting ends September 1st, take a second to vote now.
Any US Amex cardmember or US resident (who makes a guest account) can vote.
Here is the page where you can vote “nominate”
The Methuselah Foundation Page with some more details if you are interested, to vote though you only need click on the above link…
For both ethical and practical reasons, monoclonals are usually made in mice. And that’s a problem, because the human immune system recognizes the mouse proteins as foreign and sometimes attacks them instead. The result can be an allergic reaction, and sometimes even death.
To get around that problem, researchers now “humanize” the antibodies, replacing some or all of mouse-derived pieces with human ones.
Wilson and Ahmed were interested in the immune response to vaccination. Conventional wisdom held that the B-cell response would be dominated by “memory” B cells. But as the study authors monitored individuals vaccinated against influenza, they found that a different population of B cells peaked about one week after vaccination, and then disappeared, before the memory cells kicked in. This population of cells, called antibody-secreting plasma cells (ASCs), is highly enriched for cells that target the vaccine, with vaccine-specific cells accounting for nearly 70 percent of all ASCs.
“That’s the trick,” said Wilson. “So instead of one cell in 1,000 binding to the vaccines, now it is seven in 10 cells.”
All of a sudden, the researchers had access to a highly enriched pool of antibody-secreting cells, something that is relatively easy to produce in mice, but hard to come by for human B cells.
To ramp up the production and cloning of these antibodies, the researchers added a second twist. Mouse monoclonal antibodies are traditionally produced in the lab from hybridomas, which are cell lines made by fusing the antibody-producing cell with a cancer cell. But human cells don’t respond well to this treatment. So Wilson and his colleagues isolated the ASC antibody genes and transferred them into an “immortalized” cell line. The result was the generation of more than 100 different monoclonals in less than a year, with each taking just a few weeks to produce.
In the event of an emerging flu pandemic, for instance, this approach could lead to faster production of human monoclonals to both diagnose and protect against the disease.
Journal Nature article: Rapid cloning of high-affinity human monoclonal antibodies against influenza virus
Nature 453, 667–671 (29 May 2008) | doi:10.1038/nature06890; Received 16 October 2007; Accepted 4 March 2008; Published online 30 April 2008
Pre-existing neutralizing antibody provides the first line of defence against pathogens in general. For influenza virus, annual vaccinations are given to maintain protective levels of antibody against the currently circulating strains. Here we report that after booster vaccination there was a rapid and robust influenza-specific IgG+ antibody-secreting plasma cell (ASC) response that peaked at approximately day 7 and accounted for up to 6% of peripheral blood B cells. These ASCs could be distinguished from influenza-specific IgG+ memory B cells that peaked 14–21 days after vaccination and averaged 1% of all B cells. Importantly, as much as 80% of ASCs purified at the peak of the response were influenza specific. This ASC response was characterized by a highly restricted B-cell receptor (BCR) repertoire that in some donors was dominated by only a few B-cell clones. This pauci-clonal response, however, showed extensive intraclonal diversification from accumulated somatic mutations. We used the immunoglobulin variable regions isolated from sorted single ASCs to produce over 50 human monoclonal antibodies (mAbs) that bound to the three influenza vaccine strains with high affinity. This strategy demonstrates that we can generate multiple high-affinity mAbs from humans within a month after vaccination. The panel of influenza-virus-specific human mAbs allowed us to address the issue of original antigenic sin (OAS): the phenomenon where the induced antibody shows higher affinity to a previously encountered influenza virus strain compared with the virus strain present in the vaccine1. However, we found that most of the influenza-virus-specific mAbs showed the highest affinity for the current vaccine strain. Thus, OAS does not seem to be a common occurrence in normal, healthy adults receiving influenza vaccination.
With the receptor identified, a therapy can be developed that will bind to the receptor, preventing the deadly immune response. Also, by targeting a receptor in humans rather than a particular strain of flu, therapies developed to exploit this discovery would work regardless of the rapid mutations that beguile flu vaccine producers every year.
This discovery could lead to treatments which turn off the inflammation in the lungs caused by influenza and other infections, according to a study published today in the journal Nature Immunology. The virus is often cleared from the body by the time symptoms appear and yet symptoms can last for many days, because the immune system continues to fight the damaged lung. The immune system is essential for clearing the virus, but it can damage the body when it overreacts if it is not quickly contained.
The immune overreaction accounts for the high percentage of young, healthy people who died in the vicious 1918 flu pandemic. While the flu usually kills the very young or the sickly and old, the pandemic flu provoked healthy people’s stronger immune systems to react even more profoundly than usual, exacerbating the symptoms and ultimately causing between 50 and 100 million deaths world wide. These figures from the past make the new discovery that much more important, as new therapies based on this research could prevent a future H5N1 bird flu pandemic from turning into a repeat of the 1918 Spanish flu.
In the new study, the researchers gave mice infected with influenza a mimic of CD200, or an antibody to stimulate CD200R, to see if these would enable CD200R to bring the immune system under control and reduce inflammation.
The mice that received treatment had less weight loss than control mice and less inflammation in their airways and lung tissue. The influenza virus was still cleared from the lungs within seven days and so this strategy did not appear to affect the immune system’s ability to fight the virus itself.
The researchers hope that in the event of a flu pandemic, such as a pandemic of H5N1 avian flu that had mutated to be transmissible between humans, the new treatment would add to the current arsenal of anti-viral medications and vaccines. One key advantage of this type of therapy is that it would be effective even if the flu virus mutated, because it targets the body’s overreaction to the virus rather than the virus itself.
In addition to the possible applications for treating influenza, the researchers also hope their findings could lead to new treatments for other conditions where excessive immunity can be a problem, including other infectious diseases, autoimmune diseases and allergy.
What is metabolomics?
Genes are similar to the plans for a house; they show what it looks like, but not what people are getting up to inside. One way of getting a snapshot of their lives would be to rummage through their rubbish, and that is pretty much what metabolomics does. […]
Metabolomics studies metabolites, the by-products of the hundreds of thousands of chemical reactions that continuously go on in every cell of the human body. Because blood and urine are packed with these compounds, it should be possible to detect and analyse them. If, say, a tumour was growing somewhere then, long before any existing methods can detect it, the combination of metabolites from the dividing cancer cells will produce a new pattern, different from that seen in healthy tissue. Such metabolic changes could be picked up by computer programs, adapted from those credit-card companies use to detect crime by spotting sudden and unusual spending patterns amid millions of ordinary transactions.
This could be used for traditional medicine, both to prevent pathologies and to detect those that are already present so they can be treated. But another use would be as part of an early-detection system to defend against pandemics and biological attacks. As mentioned previously, network-theory can help us better use vaccines. But once you have a cure or antidote, you also need to identify people that are already infected but haven’t died yet, and the earlier you can do that after the infection, the more chances they have to live.
Once the techniques of metabolomics are sufficiently advanced and inexpensive to use, they might provide better data than simply relying on reported symptoms (might be too late by then), and might scale better than traditional detection methods (not sure yet — something else might make more economic sense). It’s a bit too early to tell, but it’s a very promising field.
For more information, see Douglas Kell’s site or Wikipedia: Metabolomics.
Source: The Economist. See also Lifeboat’s BioShield program.
If a pandemic strikes and hundreds of millions are at risk, we won’t have enough vaccines for everybody, at least not within the time window where vaccines would help. But a new strategy could help use the vaccines we have more effectively:
Researchers are now proposing a new strategy for targeting shots that could, at least in theory, stop a pandemic from spreading along the network of social interactions. Vaccinating selected people is essentially equivalent to cutting out nodes of the social network. As far as the pandemic is concerned, it’s as if those people no longer exist. The team’s idea is to single out people so that immunizing them breaks up the network into smaller parts of roughly equal sizes. Computer simulations show that this strategy could block a pandemic using 5 to 50 percent fewer doses than existing strategies, the researchers write in an upcoming Physical Review Letters.
So you break up the general social network into sub-networks, and then you target the most important nodes of these sub-networks and so on until you run out of vaccines. The challenge will be to get good information about social networks, something not quite as easy as mapping computer networks, but there is progress on that front.
In one of the most dramatic illustrations of their technique, the researchers simulated the spread of a pandemic using data from a Swedish study of social connections, in which more than 310,000 people are represented and connected based on whether they live in the same household or they work in the same place. With the new method, the epidemic spread to about 4 percent of the population, compared to nearly 40 percent for more standard strategies, the team reports.
Source: ScienceNews. See also Lifeboat’s BioShield program.
Posted in biological, chemistry, defense, existential risks, habitats, lifeboat, military, nanotechnology, nuclear weapons, sustainability | 7 Comments on $153 million/city thin film plastic domes can protect against nuclear weapons and bad weather
Cross posted from Nextbigfuture
Click for larger image
Now Alexander Bolonkin has come up with a cheaper, technological easy and more practical approach with thin film inflatable domes. It not only would provide protection form nuclear devices it could be used to place high communication devices, windmill power and a lot of other money generating uses. The film mass covered of 1 km**2 of ground area is M1 = 2×10**6 mc = 600 tons/km**2 and film cost is $60,000/km**2.
The area of big city diameter 20 km is 314 km**2. Area of semi-spherical dome is 628 km2. The cost of Dome cover is 62.8 millions $US. We can take less the overpressure (p = 0.001atm) and decrease the cover cost in 5 – 7 times. The total cost of installation is about 30–90 million $US. Not only is it only about $153 million to protect a city it is cheaper than a geosynchronous satellite for high speed communications. Alexander Bolonkin’s website
The author suggests a cheap closed AB-Dome which protects the densely populated cities from nuclear, chemical, biological weapon (bombs) delivered by warheads, strategic missiles, rockets, and various incarnations of aviation technology. The offered AB-Dome is also very useful in peacetime because it shields a city from exterior weather and creates a fine climate within the ABDome. The hemispherical AB-Dome is the inflatable, thin transparent film, located at altitude up to as much as 15 km, which converts the city into a closed-loop system. The film may be armored the stones which destroy the rockets and nuclear warhead. AB-Dome protects the city in case the World nuclear war and total poisoning the Earth’s atmosphere by radioactive fallout (gases and dust). Construction of the AB-Dome is easy; the enclosure’s film is spread upon the ground, the air pump is turned on, and the cover rises to its planned altitude and supported by a small air overpressure. The offered method is cheaper by thousand times than protection of city by current antirocket systems. The AB-Dome may be also used (height up to 15 and more kilometers) for TV, communication, telescope, long distance location, tourism, high placed windmills (energy), illumination and entertainments. The author developed theory of AB-Dome, made estimation, computation and computed a typical project.
His idea is a thin dome covering a city with that is a very transparent film 2 (Fig.1). The film has thickness 0.05 – 0.3 mm. One is located at high altitude (5 — 20 km). The film is supported at this altitude by a small additional air pressure produced by ground ventilators. That is connected to Earth’s ground by managed cables 3. The film may have a controlled transparency option. The system can have the second lower film 6 with controlled reflectivity, a further option.
The offered protection defends in the following way. The smallest space warhead has a
minimum cross-section area 1 m2 and a huge speed 3 – 5 km/s. The warhead gets a blow and overload from film (mass about 0.5 kg). This overload is 500 – 1500g and destroys the warhead (see computation below). Warhead also gets an overpowering blow from 2 −5 (every mass is 0.5 — 1 kg) of the strong stones. Relative (about warhead) kinetic energy of every stone is about 8 millions of Joules! (It is in 2–3 more than energy of 1 kg explosive!). The film destroys the high speed warhead (aircraft, bomber, wing missile) especially if the film will be armored by stone.Our dome cover (film) has 2 layers: top transparant layer 2, located at a maximum altitude (up 5 −20 km), and lower transparant layer 4 having control reflectivity, located at altitude of 1–3 km (option). Upper transparant cover has thickness about 0.05 – 0.3 mm and supports the protection strong stones (rebbles) 8. The stones have a mass 0.2 – 1 kg and locate the step about 0.5 m.
If we want to control temperature in city, the top film must have some layers: transparant dielectric layer, conducting layer (about 1 — 3 microns), liquid crystal layer (about 10 — 100 microns), conducting layer (for example, SnO2), and transparant dielectric layer. Common thickness is 0.05 — 0.5 mm. Control voltage is 5 — 10 V. This film may be produced by industry relatively cheaply.
If some level of light control is needed materials can be incorporated to control transparency. Also, some transparent solar cells can be used to gather wide area solar power.
As you see the 10 kt bomb exploded at altitude 10 km decreases the air blast effect about in 1000
times and thermal radiation effect without the second cover film in 500 times, with the second reflected film about 5000 times. The hydrogen 100kt bomb exploded at altitude 10 km decreases the air blast effect about in 10 times and thermal radiation effect without the second cover film in 20 times, with the second reflected film about 200 times. Only power 1000kt thermonuclear (hydrogen) bomb can damage city. But this damage will be in 10 times less from air blast and in 10 times less from thermal radiation. If the film located at altitude 15 km, the
damage will be in 85 times less from the air blast and in 65 times less from the thermal radiation.
For protection from super thermonuclear (hydrogen) bomb we need in higher dome altitudes (20−30 km and more). We can cover by AB-Dome the important large region and full country.
Because the Dome is light weight it could be to stay in place even with very large holes. Multiple shells of domes could still be made for more protection.
Better climate inside a dome can make for more productive farming.
AB-Dome is cheaper in hundreds times then current anti-rocket systems.
2. AB-Dome does not need in high technology and can build by poor country.
3. It is easy for building.
4. Dome is used in peacetime; it creates the fine climate (weather) into Dome.
5. AB-Dome protects from nuclear, chemical, biological weapon.
6. Dome produces the autonomous existence of the city population after total World nuclear war
and total confinement (infection) all planet and its atmosphere.
7. Dome may be used for high region TV, for communication, for long distance locator, for
astronomy (telescope).
8. Dome may be used for high altitude tourism.
9. Dome may be used for the high altitude windmills (getting of cheap renewable wind energy).
10. Dome may be used for a night illumination and entertainment
[Crossposted from the blog of Starship Reckless]
Views of space travel have grown increasingly pessimistic in the last decade. This is not surprising: SETI still has received no unambiguous requests for more Chuck Berry from its listening posts, NASA is busy re-inventing flywheels and citizens even of first-world countries feel beleaguered in a world that seems increasingly hostile to any but the extraordinarily privileged. Always a weathervane of the present, speculative fiction has been gazing more and more inwardly – either to a hazy gold-tinted past (fantasy, both literally and metaphorically) or to a smoggy rust-colored earthbound future (cyberpunk).
The philosophically inclined are slightly more optimistic. Transhumanists, the new utopians, extol the pleasures of a future when our bodies, particularly our brains/minds, will be optimized (or at least not mind that they’re not optimized) by a combination of bioengineering, neurocognitive manipulation, nanotech and AI. Most transhumanists, especially those with a socially progressive agenda, are as decisively earthbound as cyberpunk authors. They consider space exploration a misguided waste of resources, a potentially dangerous distraction from here-and-now problems – ecological collapse, inequality and poverty, incurable diseases among which transhumanists routinely count aging, not to mention variants of gray goo.
And yet, despite the uncoolness of space exploration, despite NASA’s disastrous holding pattern, there are those of us who still stubbornly dream of going to the stars. We are not starry-eyed romantics. We recognize that the problems associated with spacefaring are formidable (as examined briefly in Making Aliens 1, 2 and 3). But I, at least, think that improving circumstances on earth and exploring space are not mutually exclusive, either philosophically or – perhaps just as importantly – financially. In fact, I consider this a false dilemma. I believe that both sides have a much greater likelihood to implement their plans if they coordinate their efforts, for a very simple reason: the attributes required for successful space exploration are also primary goals of transhumanism.
Consider the ingredients that would make an ideal crewmember of a space expedition: robust physical and mental health, biological and psychological adaptability, longevity, ability to interphase directly with components of the ship. In short, enhancements and augmentations eventually resulting in self-repairing quasi-immortals with extended senses and capabilities – the loose working definition of transhuman.
Coordination of the two movements would give a real, concrete purpose to transhumanism beyond the rather uncompelling objective of giving everyone a semi-infinite life of leisure (without guarantees that either terrestrial resources or the human mental and social framework could accommodate such a shift). It would also turn the journey to the stars into a more hopeful proposition, since it might make it possible that those who started the journey could live to see planetfall.
Whereas spacefaring enthusiasts acknowledge the enormity of the undertaking they propose, most transhumanists take it as an article of faith that their ideas will be realized soon, though the goalposts keep receding into the future. As more soundbite than proof they invoke Moore’s exponential law, equating stodgy silicon with complex, contrary carbon. However, despite such confident optimism, enhancements will be hellishly difficult to implement. This stems from a fundamental that cannot be short-circuited or evaded: no matter how many experiments are performed on mice or even primates, humans have enough unique characteristics that optimization will require people.
Contrary to the usual supposition that the rich will be the first to cross the transhuman threshold, it is virtually certain that the frontline will consist of the desperate and the disenfranchised: the terminally ill, the poor, prisoners and soldiers – the same people who now try new chemotherapy or immunosuppression drugs, donate ova, become surrogate mothers, “agree” to undergo chemical castration or sleep deprivation. Yet another pool of early starfarers will be those whose beliefs require isolation to practice, whether they be Raëlians or fundamentalist monotheists – just as the Puritans had to brave the wilderness and brutal winters of Massachusetts to set up their Shining (though inevitably tarnished) City on the Hill.
So the first generation of humans adjusted to starship living are far likelier to resemble Peter Watts’ marginalized Rifters or Jay Lake’s rabid Armoricans, rather than the universe-striding, empowered citizens of Iain Banks’ Culture. Such methods and outcomes will not reassure anyone, regardless of her/his position on the political spectrum, who considers augmentation hubristic, dehumanizing, or a threat to human identity, equality or morality. The slightly less fraught idea of uploading individuals into (ostensibly) more durable non-carbon frames is not achievable, because minds are inseparable from the neurons that create them. Even if technological advances eventually enable synapse-by synapse reconstructions, the results will be not transfers but copies.
Yet no matter how palatable the methods and outcomes are, it seems to me that changes to humans will be inevitable if we ever want to go beyond the orbit of Pluto within one lifetime. Successful implementation of transhumanist techniques will help overcome the immense distances and inhospitable conditions of the journey. The undertaking will also bring about something that transhumanists – not to mention naysayers – tend to dread as a danger: speciation. Any significant changes to human physiology (whether genetic or epigenetic) will change the thought/emotion processes of those altered, which will in turn modify their cultural responses, including mating preferences and kinship patterns. Furthermore, long space journeys will recreate isolated breeding pools with divergent technology and social mores (as discussed in Making Aliens 4, 5 and 6).
On earth, all “separate but equal” doctrines have wrought untold misery and injustice, whether those segregated are genders in countries practicing Sharia, races in the American or African South, or the underprivileged in any nation that lacks decent health policies, adequate wages and humane laws. Speciation of humanity on earth bids fair to replicate this pattern, with the ancestral species (us) becoming slaves, food, zoo specimens or practice targets to our evolved progeny, Neanderthals to their Cro-Magnons, Eloi to their Morlocks. On the other hand, speciation in space may well be a requirement for success. Generation of variants makes it likelier that at least one of our many future permutations will pass the stringent tests of space travel and alight on another habitable planet.
Despite their honorable intentions and progressive outlook, if the transhumanists insist on first establishing a utopia on earth before approving spacefaring, they will achieve either nothing or a dystopia as bleak as that depicted in Paolo Bacigalupi’s unsparing stories. If they join forces with the space enthusiasts, they stand a chance to bring humanity through the Singularity some of them so fervently predict and expect – except it may be a Plurality of sapiens species and inhabited worlds instead.
The Economist has a piece on the Global Viral Forecasting Initiative (GVFI):
Dr [Nathan] Wolfe, [a virologist at the University of California, Los Angeles], is attempting to create what he calls the Global Viral Forecasting Initiative (GVFI). This is still a pilot project, with only half a dozen sites in Africa and Asia. But he hopes, if he can raise the $50m he needs, to build it into a planet-wide network that can forecast epidemics before they happen, and thus let people prepare their defences well in advance. […]
The next stage of the project is to try to gather as complete an inventory as possible of animal viruses, and Dr Wolfe has enlisted his hunters to take blood samples from whatever they catch. He is collaborating with Eric Delwart and Joe DeRisi of the University of California, San Francisco, to screen this blood for unknown viral genes that indicate new species. The GVFI will also look at people, monitoring symptoms of ill health of unknown cause and trying to match these with unusual viruses.
More here. See also the Lifeboat Foundation’s BioShield program.
Today, the University of Colorado at Boulder made an announcement regarding a very promising technology:
Known as optical frequency comb spectroscopy, the technique is powerful enough to sort through all the molecules in human breath and sensitive enough to distinguish rare molecules that may be biomarkers for specific diseases
Combined with other rapid-response technologies, this could be part of the detection side of a BioShield, a technological immune system for humanity.
The optical frequency comb is a very precise laser for measuring different colors, or frequencies, of light, said Ye. Each comb line, or “tooth,” is tuned to a distinct frequency of a particular molecule’s vibration or rotation, and the entire comb covers a broad spectral range — much like a rainbow of colors — that can identify thousands of different molecules.
Cross posted from Next big future
Since a journal article was submitted to the Royal Society of Chemistry, the U of Alberta researchers have already made the processor and unit smaller and have brought the cost of building a portable unit for genetic testing down to about $100 Cdn. In addition, these systems are also portable and even faster (they take only minutes). Backhouse, Elliott and McMullin are now demonstrating prototypes of a USB key-like system that may ultimately be as inexpensive as standard USB memory keys that are in common use – only tens of dollars. It can help with pandemic control and detecting and control tainted water supplies.
This development fits in with my belief that there should be widespread inexpensive blood, biomarker and genetic tests to help catch disease early and to develop an understanding of biomarker changes to track disease and aging development. We can also create adaptive clinical trials to shorten the development and approval process for new medical procedures
The device is now much smaller than size of a shoe-box (USB stick size) with the optics and supporting electronics filling the space around the microchip
Canadian scientists have succeeded in building the least expensive portable device for rapid genetic testing ever made. The cost of carrying out a single genetic test currently varies from hundreds to thousands of pounds, and the wait for results can take weeks. Now a group led by Christopher Backhouse, University of Alberta, Edmonton, have developed a reusable microchip-based system that costs just 500 (pounds) to build, is small enough to be portable, and can be used for point-of-care medical testing.
To keep costs down, ‘instead of using the very expensive confocal optics systems currently used in these types of devices we used a consumer-grade digital camera’, Backhouse explained.
The device can be adapted for used in many different genetic tests. ‘By making small changes to the system you could test for a person’s predisposition to cancer, carry out pharmacogenetic tests for adverse drug reactions or even test for pathogens in a water supply,’ said Backhouse.
The heart of the unit, the ‘chip,’ looks like a standard microscope slide etched with fine silver and gold lines. That microfabricated chip applies nano-biotechnologies within tiny volumes, sometimes working with only a few molecules of sample. Because of this highly integrated chip (containing microfluidics and microscale devices), the remainder of the system is inexpensive ($1,000) and fast.
There are many possible uses for such a portable genetic testing unit:
Backhouse notes that adverse drug reactions are a major problem in health care. By running a quick genetic test on a cancer patient, for example, doctors might pinpoint the type of cancer and determine the best drug and correct dosage for the individual.
Or health-care professionals can easily look for the genetic signature for a virus or E. coli – also making it useful for testing water quality.
“From a public health point of view, it would be wonderful during an epidemic to be able to do a quick test on a patient when they walk into an emergency room and be able to say, ‘you have SARS, you need to go into that (isolation) room immediately.’ ”
A family doctor might determine a person’s genetic predisposition to an illness during an office visit and advise the patient on preventative lifestyle changes.
FURTHER READING
Microfabrication technologies research at the University of Alberta
In collaboration with the Glerum Lab we have been developing microchip based implementations of genetic amplification (PCR — the polymerase chain reaction) and capillary electrophoresis (CE) that are extremely fast.