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News of Note—Changing multiple genes with CRISPR; Accelerating recovery after chemo and radiation

The evolving gene-editing technology CRISPR-Cas9 is useful for changing one gene, or maybe a few genes at a time. A team at ETH Zurich has tweaked the technology so they can change 25 different gene sites at once. Instead of using the Cas9 enzyme to do the DNA cutting, though, they used Cas12a. That allowed them to create a long “address list” of gene sites to target, they explained in the journal Nature Methods. They created a DNA molecule called a plasmid to store the list, inserted it in human cells and were able to modify several genes, they reported. (Release)

Chemotherapy and radiation suppress blood stem cells, often for several weeks or even months after cancer treatments are complete. This leaves patients vulnerable to infections and other health problems. Scientists at the University of California, Los Angeles have created a new drug that targets the protein tyrosine phosphatase-sigma (PTP-sigma), which is prevalent on blood stem cells. They showed that blocking the protein in rodent models with the drug, called DJ009, helped blood cells recover more quickly after they were damaged by radiation. They published their findings in the journal Nature Communications. (Release)

The Woman Who Gave Us the Science of Normal Life

Ellen Swallow Richards was not going to be intimidated by a room full of health experts and officials. Children were dying and their parents, Boston’s taxpayers, and city officials were to blame. The tiny, square-chinned woman thought nothing of climbing over boulders in petticoats, collecting thousands of water samples by horseback, or exploring mines on her honeymoon. So when she took the podium at the 1896 meeting of the American Public Health Association, she wasted no time in laying out her evidence.

More than 5,000 cases of illness could be attributed to the illegal conditions in Boston’s public schools, she said. Buildings lacked ventilation. Sewer pipes were still open. Toilets were filthy. Some 41 percent of the floors had never been washed. Only 27 of the city’s 168 schools had fire escapes that worked. Fully half of Boston’s schoolhouses were “deleterious to health.” The public and parents should be charged with “the murder of some 200 children per year,” Richards declared, their deaths entirely preventable from environmental hazards.

The strident, accusatory tone of Richards’ speech was remarkable, given how tactful she had been in the first two decades of her career. That tact had been a coping strategy, characteristic of a pragmatic feminism. Richards had been the first woman admitted to the Massachusetts Institute of Technology and the university’s first woman instructor. To blend in, she made a conscious effort to appear as unthreatening and feminine as she possibly could to her male colleagues. She even mended their clothes when they asked.

Stopping Pandemic X: DARPA Names Researchers Working to Halt Outbreaks

The Defense Advanced Research Projects Agency (DARPA) launched the Pandemic Prevention Platform (P3) program in 2017, with the eventual goal of halting the spread of any infectious disease outbreak before it can escalate into a pandemic.

Current approaches for recent public health emergencies due to infectious diseases have not produced effective preventive or therapeutic solutions in a relevant timescale. Examples from recent outbreaks such as H3N2 (flu), Ebola, and Zika viruses highlight the significant lag in deployment and efficacy of life-saving solutions.

A.I. Is Learning From Humans. Many Humans

Before an A.I. system can learn, someone has to label the data supplied to it. Humans, for example, must pinpoint the polyps. The work is vital to the creation of artificial intelligence like self-driving cars, surveillance systems and automated health care.


Artificial intelligence is being taught by thousands of office workers around the world. It is not exactly futuristic work.

At iMerit offices in Kolkata, India, employees label images that are used to teach artificial intelligence systems. Credit Credit Rebecca Conway for The New York Times.

Tweaked CRISPR in neurons gives scientists new power to probe brain diseases

A team of scientists at UC San Francisco and the National Institutes of Health have achieved another CRISPR first, one which may fundamentally alter the way scientists study brain diseases.

In a paper published August 15 in the journal Neuron, the researchers describe a technique that uses a special version of CRISPR developed at UCSF to systematically alter the activity of in human neurons generated from , the first successful merger of stem cell-derived cell types and CRISPR screening technologies.

Though mutations and other genetic variants are known to be associated with an increased risk for many , technological bottlenecks have thwarted the efforts of scientists working to understand exactly how these genes cause .

Dr. Denise Montell — UC Santa Barbara — Department of Molecular, Cellular, and Developmental Biology — Anastasis — ideaXme — Ira Pastor

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