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Category: biotech/medical – Page 873

Researchers developed a new method called wildDISCO that uses standard antibodies to map the entire body of an animal using fluorescent markers. This revolutionary technique provides detailed 3D maps of structures, shedding new light on complex biological systems and diseases. WildDISCO has the potential to transform our understanding of intricate processes in health and disease and paves the way for exciting advancements in medical research. This technology was now introduced in Nature Biotechnology.

In the past, scientists relied on genetically modified animals or specialized labels to make specific structures and cells of interest visible in the entire body of an animal. But these approaches are expensive and time-consuming to create, especially when it comes to body-wide systems such as the nervous system.

A team of scientists from Helmholtz Munich, the LMU University Hospital and the Ludwig-Maximilians Universität München (LMU) now introduced a new method called wildDISCO, which makes use of standard antibodies to map whole bodies of mice. This ultimately enables the creation of detailed three-dimensional maps of normal and diseased structures in mammalian bodies in an easy-to-use and cost-efficient way.

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This was a surprise. Animals have brain maps for vision and touch, but these are built from visual images and touch receptors that map onto the brain through direct point‑to‑point projections. With ears, it’s entirely different. The brain compares information received from each ear about the timing and intensity of a sound and then translates the differences into a unified perception of a single sound issuing from a specific region of space. The resulting auditory map allows owls to “see” the world in two dimensions with their ears.

This proved to be a big leap toward understanding how the brain of any animal, including humans, learns to grasp its environment through sound. Think of it. Standing in a forest, you hear the crack of a falling branch or the rustle of a deer’s step in the dry leaves. Your brain calculates the time and intensity of sound to determine where it’s coming from. Owls do this task with incredible speed and accuracy. Each cochlea in the owl provides the brain with the precise timing of the sound reaching that ear within 20 microseconds. This determines how accurately the brain can calculate the interaural time difference, which in turn determines the accuracy of the localization of a sound in the azimuth. “The precision in microseconds provided by the owl cochlea is better than in any other animal that has been tested,” says Köppl. “We have big heads, so the interaural time differences are larger, making the task for cochlea and brain easier. In a nutshell, it is the combination of a small head and very precise localization that makes the owl unique.”

And here’s a finding to drop the jaw. José Luis Peña, a neuroscientist at the Albert Einstein College of Medicine, and his collaborators have discovered that the sound localization system in a barn owl’s brain performs sophisticated mathematical computations to execute this pinpointing of prey. The space‑specific neurons in the owl’s specialized auditory brain do advanced math when they transmit their information, not just adding and multiplying incoming signals but averaging them and using a statistical method called “Bayesian inference,” which involves updating as more information becomes available.

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That experiences leave their trace in the connectivity of the brain has been known for a while, but a pioneering study by researchers at the German Center for Neurodegenerative Diseases (DZNE) and TUD Dresden University of Technology now shows how massive these effects really are. The findings in mice provide unprecedented insights into the complexity of large-scale neural networks and brain plasticity. Moreover, they could pave the way for new brain-inspired artificial intelligence methods. The results, based on an innovative “brain-on-chip” technology, are published in the scientific journal Biosensors and Bioelectronics.

The Dresden researchers explored the question of how an enriched experience affects the brain’s circuitry. For this, they deployed a so-called neurochip with more than 4,000 electrodes to detect the electrical activity of brain cells. This innovative platform enabled registering the “firing” of thousands of neurons simultaneously. The area examined – much smaller than the size of a human fingernail – covered an entire mouse hippocampus. This brain structure, shared by humans, plays a pivotal role in learning and memory, making it a prime target for the ravages of dementias like Alzheimer’s disease. For their study, the scientists compared brain tissue from mice, which were raised differently. While one group of rodents grew up in standard cages, which did not offer any special stimuli, the others were housed in an “enriched environment” that included rearrangeable toys and maze-like plastic tubes.

“The results by far exceeded our expectations,” said Dr. Hayder Amin, lead scientist of the study. Amin, a neuroelectronics and nomputational neuroscience expert, heads a research group at DZNE. With his team, he developed the technology and analysis tools used in this study. “Simplified, one can say that the neurons of mice from the enriched environment were much more interconnected than those raised in standard housing. No matter which parameter we looked at, a richer experience literally boosted connections in the neuronal networks. These findings suggest that leading an active and varied life shapes the brain on whole new grounds.”

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With triplex origami, scientists can achieve a level of artificial control over the shape of double-stranded DNA that was previously unimaginable, thereby opening new avenues of exploration, according to the Aarhus University researchers. It has recently been suggested that triplex formation plays a role in the natural compaction of genetic DNA and the current study may offer insight into this fundamental biological process.

Potential in gene therapy and beyond

The work also demonstrates that the Hoogsteen-mediated triplex formation shields the DNA against enzymatic degradation. Thus, the ability to compact and protect DNA with the triplex origami method may have large implications for gene therapy, wherein diseased cells are repaired by encoding a function that they are missing into a deliverable piece of double-stranded DNA.

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The process of converting DNA to proteins through an RNA is far from straightforward. Of the several types of RNA involved in the process of protein synthesis, a few may be edited mid-way. In mammals, RNA editing mostly involves converting adenosine (A) to inosine (I) through deamination, which can result in a wide range of effects. For example, A-to-I conversion can regulate gene expression in different ways and significantly alter the final synthesized protein.

While RNA editing is an essential biological process, it is also a key underlying mechanism in some diseases, including cancer. Thus, scientists have created large-scale databases documenting RNA editing sites in various human tissues. These databases serve as useful platforms for identifying potential diagnostic or therapeutic targets from the RNA editome, which encompasses all edited RNA molecules in a given cell or tissue.

Unfortunately, there are currently no databases for RNA editing in hematopoietic cells. The hematopoietic cells are unique in that they can develop into all types of cells including , , and platelets.

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Like its viral cousins, a somewhat parasitic DNA sequence called a retrotransposon has been found borrowing the cell’s own machinery to achieve its goals.

In a new work appearing online Wednesday in the journal Nature, a Duke University team has determined that retrotransposons hijack a little-known piece of the cell’s DNA repair function to close themselves into a ring-like shape and then create a matching double strand.

The finding upends 40 years of conventional wisdom saying these rings were just a useless by-product of bad gene copying. It may also offer new insights into cancer, viral infections and immune responses.

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For cellular agriculture—a technique that grows meat in bioreactors—to successfully feed millions, numerous technological hurdles must be conquered. The production of muscle cells from sources such as chicken, fish, cows, and more will need to increase to the point where millions of metric tons are yielded annually.

Researchers at the Tufts University Center for Cellular Agriculture (TUCCA) have made strides toward this objective by developing immortalized bovine muscle stem cells (iBSCs). These cells possess a rapid growth rate and the ability to divide hundreds of times, potentially even indefinitely, furthering the potential for large-scale meat production.

This advance, described in the journal ACS Synthetic Biology, means that researchers and companies around the globe can have access to and develop new products without having to source cells repeatedly from farm animal biopsies.

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Sports teams spend millions of dollars on their players’ health and fitness and any injuries can be detrimental to their players’ careers. Artificial intelligence (AI) has the potential to significantly change the way that sports spine injuries are diagnosed, treated, and managed. Tools such as Spindle and SpindleX are making it easy to prevent long-term injuries or spinal issues by detecting even the minutest variations in time. However, AI has just begun its foray into the field of healthcare and more importantly radiology or spine imaging.

With AI-related radiology imaging, it is becoming easier to prevent and cure injuries we didn’t even know existed. AI-assisted reports are helping physicians and surgeons take better and more accurate decisions and treatment plans, saving millions of dollars in the healthcare industry. Here are a few examples of how AI is improving the treatment of sports-related spine injuries:

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Initial staging of prostate cancer (PCa) is usually performed with conventional imaging (CI), involving computed tomography (CT) and bone scanning (BS). The aim of this study was to analyze the role of [18F]F-choline positron emission tomography (PET)/CT in the initial management and outcome prediction of PCa patients by analyzing data from a multidisciplinary approach. We retrospectively analyzed 82 patients who were discussed by the uro-oncology board of the University Hospital of Ferrara for primary staging newly diagnosed PCa (median age 72 (56–86) years; median baseline prostate specific antigen (PSA) equal to 8.73 ng/mL). Patients were divided into three groups based on the imaging performed: group A = only CI; group B = CI + [18F]F-choline PET/CT; group C = only [18F]F-choline PET/CT. All data on imaging findings, therapy decisions and patient outcomes were retrieved from hospital information systems. Moreover, we performed a sub-analysis of semiquantitative parameters extracted from [18F]F-choline PET/CT to search any correlation with patient outcomes. The number of patients included in each group was 35, 35 and 12, respectively. Patients with higher values of initial PSA were subjected to CI + PET/CT (p = 0.005). Moreover, the use of [18F]F-choline PET/CT was more frequent in patients with higher Gleason score (GS) or ISUP grade (p = 0.013). The type of treatment performed (surgery n = 33; radiation therapy n = 22; surveillance n = 6; multimodality therapy n = 6; systemic therapy n = 13; not available n = 2) did not show any relationship with the modality adopted to stage the disease. [18F]F-choline PET/CT induced a change of planned therapy in 5/35 patients in group B (14.3%). Moreover, patients investigated with [18F]F-choline PET/CT alone demonstrated longer biochemical recurrence (BCR)-free survival (30.8 months) in comparison to patients of groups A and B (15.5 and 23.5 months, respectively, p = 0.006), probably due to a more accurate selection of primary treatment. Finally, total lesion choline kinase activity (TLCKA) of the primary lesion, calculated by multiplying metabolic tumor volume and mean standardized uptake value (SUVmean), was able to more effectively discriminate patients who had recurrence after therapy compared to those without (p = 0.03). In our real-world experience [18F]F-choline PET/CT as a tool for the initial management of PCa had a relevant impact in terms of therapy selection and was associated with longer BCR-free survival. Moreover, TLCKA of the primary lesion looks a promising parameter for predicting recurrence after curative therapy.

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The innate immune system is able to identify foreign invaders and immediately respond to them. This system is important in order to protect the body from harmful substances.

The response to an infection triggers the arrival of cells called neutrophils, which attack the infection, followed by macrophages that attack bacteria and viruses.

Macrophages release cytokines in order to communicate with other cells when they encounter an enemy. Cytokines are small proteins that carry information. The immune system is activated by cytokines, which give the immune cells direction to fight.

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