Lifeboat Foundation

Biologists often speak of switching genes on and off to give microbes new abilities–like producing biofuels or drugs, or gobbling up environmental toxins. For the most part, though, it’s nearly impossible to turn off a gene without deleting it (which means you can’t turn it on again). This limits biologists’ ability to control how much of a particular protein a microbe produces. It also restricts bioengineers’ ability to design new microbes.

Now researchers at Boston University, led by biomedical engineering professor James Collins, have developed a highly tunable genetic “switch” that offers a greater degree of control over microbes. It makes it possible to stop the production of a protein and restart it again. The switch, which could be used to control any gene, can also act as a “dimmer switch” to finely tune how much protein a microbe would produce over time.

The researchers made a highly effective microbe “kill switch” to demonstrate the precision of the approach. For years, researchers have been trying to develop these self-destruction mechanisms to allay concerns that genetically engineered microbes might prove impossible to eradicate once they’ve outlived their usefulness. But previous kill switches haven’t offered tight enough control to pass governmental regulatory muster because it was difficult to make it turn on in all the cells in a population at the same time.

We are witnessing the birth of a new faith. It is not a theistic religion. Indeed, unlike Christianity, Judaism, and Islam, it replaces a personal relationship with a transcendent God in the context of a body of believers with a fervent and radically individualistic embrace of naked materialistic personal recreation.

Moreover, in contrast to the orthodox Christian, Judaic, and Islamic certainty that human beings are made up of both material body and immaterial soul – and that both matter – adherents of the new faith understand that we have a body, but what really counts is mind, which is ultimately reducible to mere chemical and electrical exchanges. Indeed, contrary to Christianity’s view of an existing Heaven or, say, Buddhism’s conception of the world as illusion, the new faith insists that the physical is all that has been, is, or ever will be.

Such thinking leads to nihilism. That’s where the new religion leaves past materialistic philosophies behind, by offering adherents hope. Where traditional theism promises personal salvation, the new faith offers the prospect of rescue via radical life-extension attained by technological applications – a postmodern twist, if you will, on faith’s promise of eternal life. This new religion is known as “transhumanism,” and it is all the rage among the Silicon Valley nouveau riche, university philosophers, and among bioethicists and futurists seeking the comforts and benefits of faith without the concomitant responsibilities of following dogma, asking for forgiveness, or atoning for sin – a foreign concept to transhumanists. Truly, transhumanism is a religion for our postmodern times.

“I HOPE THIS STUDY CHANGES THE DIALOG AROUND HERPES RESEARCH AND OPENS UP THE IDEA THAT WE CAN START THINKING ABOUT CURE, RATHER THAN JUST CONTROL OF THE VIRUS.”

In a landmark study, researchers have successfully used gene editing to remove the oral herpes virus (HSV-1) in mice.

While previous research has mostly focused on treating and suppressing the sometimes painful symptoms of herpes, this study took a more radical approach by attempting to eliminate the virus altogether.

Continue reading “Scientists Use Gene-Hacking to Seemingly Cure Herpes in Mice” »

Scientists hope the CRISPR-based therapy could treat neurodegenerative disease.

The terrorist or psychopath of the future, however, will have not just the Internet or drones—called “slaughterbots” in this video from the Future of Life Institute—but also synthetic biology, nanotechnology, and advanced AI systems at their disposal. These tools make wreaking havoc across international borders trivial, which raises the question: Will emerging technologies make the state system obsolete? It’s hard to see why not. What justifies the existence of the state, English philosopher Thomas Hobbes argued, is a “social contract.” People give up certain freedoms in exchange for state-provided security, whereby the state acts as a neutral “referee” that can intervene when people get into disputes, punish people who steal and murder, and enforce contracts signed by parties with competing interests.

The trouble is that if anyone anywhere can attack anyone anywhere else, then states will become—and are becoming—unable to satisfy their primary duty as referee.

Continue reading “Omniviolence Is Coming and the World Isn’t Ready” »

Washington State University researchers have made a key advance in solid oxide fuel cells (SOFCs) that could make the highly energy-efficient and low-polluting technology a more viable alternative to gasoline combustion engines for powering cars.

Led by Ph.D. graduate Qusay Bkour and Professor Su Ha in the Gene and Linda Voiland School of Chemical Engineering and Bioengineering, the researchers have developed a unique and inexpensive nanoparticle catalyst that allows the fuel cell to convert logistic liquid fuels such as gasoline to electricity without stalling out during the electrochemical process. The research, featured in the journal, Applied Catalysis B: Environmental, could result in highly efficient gasoline-powered cars that produce low carbon dioxide emissions that contribute to global warming.

“People are very concerned about energy, the environment, and global warming,” said Bkour. “I’m very excited because we can have a solution to the energy problem that also reduces the emissions that cause global warming.”

The Stanford team worked with researchers at the Department of Energy’s Lawrence Berkeley National Laboratory to develop a technique called prophylactic antiviral CRISPR in human cells, or PAC-MAN. The technology disables viruses by scrambling their genetic code. The researchers developed a new way to deliver the technology into lung cells, they reported in the journal Cell.

Stanford bioengineers teamed up with researchers at the Lawrence Berkeley National Laboratory to develop a CRISPR system that neutralizes SARS-CoV-2 by scrambling the virus’s genetic code. They believe the technology could prove useful for combating several types of viruses, including influenza.

Within a mere eight years, CRISPR-Cas9 has become the go-to genome editor for both basic research and gene therapy. But CRISPR-Cas9 also has spawned other potentially powerful DNA manipulation tools that could help fix genetic mutations responsible for hereditary diseases.

Researchers at the University of California, Berkeley, have now obtained the first 3D structure of one of the most promising of these tools: base editors, which bind to DNA and, instead of cutting, precisely replace one nucleotide with another.

First created four years ago, base editors are already being used in attempts to correct single-nucleotide mutations in the human genome. Base editors now available could address about 60% of all known genetic diseases—potentially more than 15,000 inherited disorders—caused by a mutation in only one nucleotide.

Plant viruses infect many economically important crops, including wheat, cotton, maize, cassava, and other vegetables. These viruses pose a serious threat to agriculture worldwide, as decreases in cropland area per capita may cause production to fall short of that required to feed the increasing world population. Under these circumstances, conventional strategies can fail to control rapidly evolving and emerging plant viruses. Genome-engineering strategies have recently emerged as promising tools to introduce desirable traits in many eukaryotic species, including plants. Among these genome engineering technologies, the CRISPR (clustered regularly interspaced palindromic repeats)/CRISPR-associated 9 (CRISPR/Cas9) system has received special interest because of its simplicity, efficiency, and reproducibility. Recent studies have used CRISPR/Cas9 to engineer virus resistance in plants, either by directly targeting and cleaving the viral genome, or by modifying the host plant genome to introduce viral immunity. Here, we briefly describe the biology of the CRISPR/Cas9 system and plant viruses, and how different genome engineering technologies have been used to target these viruses. We further describe the main findings from recent studies of CRISPR/Cas9-mediated viral interference and discuss how these findings can be applied to improve global agriculture. We conclude by pinpointing the gaps in our knowledge and the outstanding questions regarding CRISPR/Cas9-mediated viral immunity.

Keywords: plant virus, CRISPR/Cas9, genome engineering, geminivirus, virus resistance.

In the context of the rapidly growing global population, food security has emerged as one of the major challenges facing our generation (Cheeseman, 2016). The global population has increased by 60%, but per capita production of grains has fallen worldwide in the last 20 years (Suweis et al., 2015). If the population growth rate, which is 1.13 percent per year for 2016¹ persists, the world population will double again within a mere 50 years, and it is estimated that food production will need to at least double till 2050 to meet demand (Suweis et al., 2015). Increases in food production per unit of land have not kept pace with increases in population and cropland area per capita has fallen by more than half since 1960 (Cheeseman, 2016).