Whole-brain cell mapping using AI

The researchers developed a highly multiplexed whole-mount staining technique, utilizing the repeated application of fluorescence in situ hybridization.

The technique called mFISH3D for multiplexed mRNA staining in whole mouse organs and human tissue.

The technique helps to visualize 10 types of mRNAs in an intact mouse brain.

This workflow provides a robust approach to studying selective cell vulnerability in disease. sciencenewshighlights ScienceMission https://sciencemission.com/Artificial-intelligence-driven-wh…ll-mapping

Murakami et al. developed mFISH3D for multiplexed mRNA staining in whole-mouse organs and human tissue. Analysis of the stained mouse brains using the AI-driven ZenCell platform reveals unique cell populations activated by pharmacological perturbation. This workflow provides a robust approach to studying selective cell vulnerability in disease.