Integrated genomic and transcriptomic profiling of glioblastoma reveals ecDNA-driven heterogeneity and microenvironmental reprogramming.

Tang et al. provide a comprehensive genomic and transcriptomic characterization of extrachromosomal DNA (ecDNA) in glioblastoma. They reveal that EGFR ecDNA shapes transcriptional subtypes and reprograms the tumor microenvironment by stabilizing metabolically active tumor-associated macrophages. These findings uncover mechanistic links between ecDNA architecture and glioblastoma progression, highlighting potential therapeutic vulnerabilities.