Revvity’s Dharmacon All-in-one lentiviral platform has expanded to include whole-genome library options for CRISPR knockout (CRISPRko), CRISPR interference (CRISPRi), and CRISPR activation (CRISPRa). Like the individual All-in-one reagents, the whole-genome libraries utilize a single vector lentiviral system with all components necessary for CRISPRko, CRISPRa, and CRISPRi whole-genome pooled screening.