A useful review on the nucleic acid contaminants found in AAVs, how to detect such contaminants, their biological implications, and how we can minimize nucleic acid contaminants in the future through new manufacturing innovations! #biotech #genetherapy

Brimble and colleagues review nucleic acid heterogeneity of rAAV for gene therapy. Alongside the intended expression cassette, non-therapeutic DNA is present within rAAV preparations. These contaminants can be transferred and can even expressed after delivery. They discuss sources of DNA contamination in rAAV and highlight existing strategies to improve vector purity.