Until now, only expensive and slow electron microscopes could reach this level of detail. But LICONN opens the door for more labs around the world to explore the brain’s cellular “wiring diagram” using tools they already have. It’s like giving everyone a high-powered zoom lens for decoding how the brain works, learns, and perhaps breaks down in disease.

In collaboration with the Institute of Science and Technology Austria (ISTA), we published in Nature the first-ever method for using light microscopy to comprehensively map all the neurons and their connections in a block of brain tissue. The key finding from this validation experiment is that this approach works as well as electron microscopy-based connectomics.