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CRISPR-Cas9 is an efficient and versatile tool for genome engineering in many species. However, inducible CRISPR-Cas9 editing systems that regulate Cas9 activity or sgRNA expression often suffer from significant limitations, including reduced editing capacity, off-target effects, or leaky expression. Here, we develop a precisely controlled sgRNA expression cassette that can be combined with widely-used Cre systems, termed CRISPR-Switch (SgRNA With Induction/Termination by Cre Homologous recombination). Switch-ON facilitates controlled, rapid induction of sgRNA activity. In turn, Switch-OFF-mediated termination of editing improves generation of heterozygous genotypes and can limit off-target effects. Furthermore, we design sequential CRISPR-Switch-based editing of two loci in a strictly programmable manner and determined the order of mutagenic events that leads to development of glioblastoma in mice. Thus, CRISPR-Switch substantially increases the versatility of gene editing through precise and rapid switching ON or OFF sgRNA activity, as well as switching OVER to secondary sgRNAs.

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Do you know why only Vitamin C in the form of L-ascorbic acid is truly effective against cancer?

The Molecular Structure of Ascorbic Acid – MasterKey to Health & Disease

Most living organisms including plants, insects and animals produce ascorbic acid. Ascorbic acid exists naturally in the form of L-ascorbic acid [24]. In physiological pH, L-ascorbic acid exists predominantly in the ionic form of L-ascorbate.

In their 2019 landmark study, Osipyants et al. not only showed that prolyl hydroxylase depended upon ascorbic acid as substrate, but demonstrated definitively for the first time that ascorbic acid MUST BE IN THE SPECIFIC MOLECULAR STRUCTURE of L-ASCORBATE in order to suppress HIF-1a [25]!!

Continue reading “Vitamin C & Cancer — Health & Disease Masterkey (Part 3)” | >

This sounds like a roundabout way of saying that the uploaded minds might be seen as superhuman, even though, as Minerva and Rorheim observe, “it’s not clear whether you would survive in any meaningful sense if you were copied several times over.” If you didn’t, something that used to be you might exist and be cognitively superior to the common run of humanity but the “you-ness” is gone.

The authors also warn, “We can be pretty certain, for instance, that rejuvenation would widen the gap between the rich and poor, and would eventually force us to make decisive calls about resource use, whether to limit the rate of growth of the population, and so forth.”

Such considerations as limiting the “rate of growth” of the population may relate to a controversy in 2012 involving a paper written by Dr. Minerva and Dr. Alberto Giubilini:

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This statistic displays the number of organ transplant candidates in the United States by organ, as of as of September 25, 2019. At this moment, there were 230 candidates in the country waiting for an intestine donation. Organ donation can be given through both a deceased and living donor if blood and oxygen are flowing through the organs until the time of recovery to ensure viability. There are over 120,000 people in the country waiting for an organ transplant.

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Before his death, Stephen Hawking submitted a research paper that predicts the end of the world.

Just two weeks before his death, renowned physicist Stephen Hawking submitted a research paper that suggests parallel universes and predicts the end of this one.

Hawking and his co-author Thomas Hertog published their research in “A Smooth Exit from Eternal Inflation,” detailing how scientists may also be able to detect other universes using a spaceship. According to Hertog, Hawking finished writing the paper from his deathbed, leaving behind a final legacy that is worthy of the Nobel Prize.

“He has often been nominated for the Nobel and should have won it. Now he never can,” he told the Sunday Times.

Continue reading “Stephen Hawking’s Final Research Paper Predicted the End of the Universe” | >

While propeller planes certainly do have their place, sometimes the extra speed and thrust of a jet engine is what’s really needed. Dallas, Texas-based FusionFlight has applied that sort of thinking to quadcopter-style drones, resulting in the AB5 JetQuad.

According to the company, the AB5 is “the world’s smallest and most powerful jet-powered drone with vertical take-off and landing [VTOL] capabilities.”

Instead of the usual four electric motors and propellers, the current prototype has four diesel-powered microturbine jet engines which produce a combined 200 horsepower (149 kW) at full throttle. Thanks to a proprietary vectoring system known as the H-Configuration, the thrust from these engines can be directed either to move the drone vertically when taking off and landing, or horizontally while in flight.

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