Lifeboat Foundation

Scientists have categorized different types of CRISPR systems into two classes based on how their Cas nucleases function. In class 1 (types I, III, and IV), different Cas proteins form a complex machinery to identify and cut foreign DNA; in class 2 CRISPR systems (types II, V, and VI), a single Cas protein effector recognizes and cleaves DNA.⁹

After characterizing CRISPR’s role as a defense mechanism in bacteria, researchers soon realized that they could harness this system for gene manipulation in any cell. All they needed to do was design a CRISPR gRNA sequence that bound to a specific DNA sequence and triggered the Cas nuclease, which would then cut precisely at that location. With CRISPR, researchers routinely knock out gene function by cutting out a DNA fragment, or they insert a desired genetic sequence into the genome by providing a reference DNA template along with the CRISPR components. While editing eukaryotic cells has been the focus for tackling diseases, many researchers now use CRISPR to edit bacterial communities.

“It’s almost like back to the beginning or back to the origins. There’s some irony in bringing CRISPR back to where it came from,” said Rodolphe Barrangou, a functional genomics researcher at North Carolina State University, who helped characterize the immune function of CRISPR and has been working with it for more than 20 years.

Anyone know anything else? This is a supplement easily found too.

Dr. Stephanie Venn-Watson presents a newly discovered natural molecule that has potential benefits to extend both healthspan \& lifespan in this video.

Continue reading “THIS NOVEL Natural Longevity Gamechanger — Outperforming Top Molecules in the Field??” »

What if AI companies like Chat GPT were actually honest about how horrifically terrible they are? Roger Horton investigates.

SUBSCRIBE HERE: http://goo.gl/ITTCPW

Continue reading “If Artificial Intelligence Was Honest | Honest Ads [ChatGPT, A.I. Parody]” »

Wired writer Mark Andrews tested three Chinese vehicles equipped with semi-autonomous functionality and found them superior to comparable American self-driving systems. The reasons, it seems, boil down to a single feature that American passenger cars have yet to implement: Lidar. From Wired:

On the flip side, Tesla and General Motors have been grabbing most of the recent headlines when it comes to self-driving cars in the hands of the public, and for all the wrong reasons—mass recalls, suspended licenses, spending cuts, and huge losses.

But in China, a number of companies are steadily—and far more successfully—moving toward a similar destination, but via a different route.

The first thing many media seem not to understand is the methodology followed by Space X, which is completely different from what the traditional aerospace builders do. While the latter prefer to spend their money on a long project life cycle, including long requirements discussion, and meticulous and detailed test engineering and integration phases, Space X opts for a methodology closer to the experimental scientific method: draw essential requirements, build a prototype, test, fail, learn from failures, build a new improved prototype, and try again. Each reiteration adds quality to the project, up to a point when the prototype is working well, and Falcon 9 (as a sample) becomes the space workhorse with any more competitors in the world. Is that so hard to be understood, for journalists?When a traditional project fails, many billions are wasted, and many years of work are canceled. When a “normal” failure occurs during Space X’s reiterative project development, very less resources are wasted. And, after all, during the expendable rockets’ age, all the rockets were always wasted, at every launch! The difference is incomparable. Another advantage of this method is its high flexibility. If a project lasts 10 years, it is difficult to take advantage of the technological advances: switching to new technology in a project initiated many years ago forces heavy requirements reviews and unavoidable delays. In a fail-and-repeat project, new technologies and new ideas can be adopted more easily and more quickly, as demonstrated by the thousands of changes and improvements applied to the different starships, super-heavy boosters, and raptor engine prototypes throughout history. Despite the misfortune bearers and the envious, the methodology works. The success of Space X in the launchers market doesn’t lie.

Starship 28 and the Super-Heavy Booster 10 made most of the expected work, and even more than what was expected: while the suborbital altitude was planned, the Starship spacecraft reached 230 km, a low Earth orbit altitude at more than 26,200 km/h. several tests were conducted after the engine cutoff, including a propellant transfer demo and payload dispenser test.

Only two operations have failed. The booster couldn’t make it to descend vertically on its engines, since only 3 of them reignited, and splashed in the Mexican Gulf at little more than 1,000 km/h. The Starship failed during the re-entry in the atmosphere, in the Indian Ocean. We could observe many insulating tiles flying away from the Starship’s body during the first part of the re-entry. At an altitude of 65 km, telemetry from Ship 28 was lost, and the vehicle was destroyed before splashing in the sea.

Scientists develop anticancer drugs that target PARP proteins involved in the DNA damage response.

Why are so many young people getting cancer?

Here’s what the data say:

Clues to a modern mystery could be lurking in information collected generations ago.

Nanomedicine to Cure All!

Aortic aneurysms are bulges in the aorta, the largest blood vessel that carries oxygen-rich blood from the heart to the rest of the body. Smoking, high blood pressure, diabetes, or injury can all increase the risk of aneurysms, which tend to occur more often in Caucasian male smokers over the age of 65.

“The soft tissues that make up blood vessels act essentially like rubber bands, and it’s the elastic fibers within these tissues that allow them to stretch and snap back,” says Professor Anand Ramamurthi, chair of the Department of Bioengineering in Lehigh University’s P.C. Rossin College of Engineering and Applied Science. “These fibers are produced primarily before and just after birth. After that, they don’t regenerate or undergo natural repair after injury. So when they become injured or diseased, the tissue weakens and causes an aneurysm, which can grow over time. After about seven to 10 years, it typically reaches the rupture stage.”

Continue reading “Nanomedicine research aims to transform treatment of aortic aneurysms” »

Models of systems in physics usually start with elementary processes. New work with a neural network shows how models can also be built by observing the system as a whole and deducing the underlying interactions.