Molecular detective work is zeroing in on the origins of sexual reproduction. The protein tools for cell mergers seem to have long predated sex — so what were they doing?
Cellular reprogramming builds on the Nobel Prize-winning work of Shinya Yamanaka, who showed that adult cells could be transformed back into stem cells by exposing them to a specific set of genome-regulating proteins known as transcription factors. The Salk team’s innovation was to reduce the exposure times to the so-called Yamanaka factors, which they found could reverse epigenetic changes to the cells without reverting them to stem cells.
While the approach led to clear increases in lifespan in prematurely aging mice, the fact that no one had been able to replicate the result in healthy mice since then raised doubts about the approach. “Different groups have tried this experiment, and the data have not been positive so far,” Alejandro Ocampo, from the University of Lausanne in Switzerland, who carried out the original Salk experiments, told MIT Technology Review.
But now, Rejuvenate Bio claims that when they exposed healthy mice near the end of their lives to a subset of the Yamanaka factors, they lived for another 18 weeks on average, compared to just 9 weeks for those that didn’t undergo cellular reprogramming.
Using the James Webb Space Telescope, scientists say they’ve now observed galaxies from when the universe was just 330 million years old.
Aging is a complex process best characterized as the chronic dysregulation of cellular processes leading to deteriorated tissue and organ function. While aging cannot currently be prevented, its impact on lifespan and healthspan in the elderly can potentially be minimized by interventions that aim to return these cellular processes to optimal function. Recent studies have demonstrated that partial reprogramming using the Yamanaka factors (or a subset; OCT4, SOX2, and KLF4; OSK) can reverse age-related changes in vitro and in vivo. However, it is still unknown whether the Yamanaka factors (or a subset) are capable of extending the lifespan of aged wild type mice. Here, we show that systemically delivered AAVs, encoding an inducible OSK system, in 124-week-old mice extends the median remaining lifespan by 109% over wild-type controls and enhances several health parameters. Importantly, we observed a significant improvement in frailty scores indicating that we were able to improve the healthspan along with increasing the lifespan. Furthermore, in human keratinocytes expressing exogenous OSK, we observed significant epigenetic markers of age-reversal, suggesting a potential reregulation of genetic networks to a younger, potentially healthier state. Together, these results may have important implications for the development of partial reprogramming interventions to reverse age-associated diseases in the elderly.
All authors performed the work while employed at Rejuvenate Bio Inc. Rejuvenate Bio is a therapeutics company translating gene therapies to treat age-related diseases.
Year 2017 face_with_colon_three
Biological molecules, like organisms themselves, are subject to genetic drift and may even become “extinct”. Molecules that are no longer extant in living systems are of high interest for several reasons including insight into how existing life forms evolved and the possibility that they may have new and useful properties no longer available in currently functioning molecules. Predicting the sequence/structure of such molecules and synthesizing them so that their properties can be tested is the basis of “molecular resurrection” and may lead not only to a deeper understanding of evolution, but also to the production of artificial proteins with novel properties and even to insight into how life itself began.
Year 2013 face_with_colon_three
Francisco José Soler Gil, Manuel Alfonseca, Fine Tuning Explained? Multiverses and Cellular Automata, Journal for General Philosophy of Science / Zeitschrift für allgemeine Wissenschaftstheorie, Vol. 44, No. 1 (July 2013), pp. 153–172.
Read more about it here: https://ow.ly/jV1H50Mtv2S📷 @esa / @HUBBLE_space & @NASA M. Stiavelli, P. Erwin et al. pic.twitter.com/hprRJ8APnN
— HUBBLE (@HUBBLE_space) January 19, 2023.
Nearly a year after it began, the worst avian-influenza outbreak in U.S. history is continuing to decimate poultry flocks across the Midwest and Colorado, frustrating efforts to keep the virus from affecting the nation’s egg prices and supply.
In South Dakota, the highly contagious bird flu, typically transmitted by the feces, mucus and saliva of wild birds, first hit commercial poultry farms in March 2022 and has continued to affect flocks. Within the last month, egg-laying hens and turkeys at several local farms were infected, leading to the deaths of more than 1.3 million poultry over that period, according to U.S. Department of Agriculture data. Nearly four million poultry have died in the state since the start of the outbreak.
In 2020 I joined the private beta test of Open AI’s Generative Pre-trained Transformer 3 (GPT-3), which is an earlier version of ChatGPT. When ChatGPT was released in November 2022, I started experimenting with it. For over two years I’ve been exploring the strengths and limits of this technology and assessing how this tool could be useful to me. I’m also interested how this new technology is being utilized by scientists and academics to make meaningful contributions to academic work and education.
A recent study demonstrated that ChatGPT was able to pass the US Medical Licensing Exam without any special training prior to the exam and was able to demonstrate a high level of insight in its explanations. The results suggest that ChatGPT may be able to assist with medical education.
I published the first article about my experiments with ChatGPT last week. The article entitled How The Evolution Of AI In Healthcare Aligns With Thomas Kuhn’s Structure has been viewed over 13,000 times, and has received and
Year 2019 face_with_colon_three
In a study that raises profound questions about the line between life and death, researchers have restored some cellular activity to brains removed from slaughtered pigs.
The brains did not regain anything resembling consciousness: There were no signs indicating coordinated electrical signaling, necessary for higher functions like awareness and intelligence.
Continue reading “‘Partly Alive’: Scientists Revive Cells in Brains From Dead Pigs” »
Aging is a complex process best characterized as the chronic dysregulation of cellular processes leading to deteriorated tissue and organ function. While aging cannot currently be prevented, its impact on lifespan and healthspan in the elderly can potentially be minimized by interventions that aim to return these cellular processes to optimal function. Recent studies have demonstrated that partial reprogramming using the Yamanaka factors (or a subset; OCT4, SOX2, and KLF4; OSK) can reverse age-related changes in vitro and in vivo. However, it is still unknown whether the Yamanaka factors (or a subset) are capable of extending the lifespan of aged wild type mice. Here, we show that systemically delivered AAVs, encoding an inducible OSK system, in 124-week-old mice extends the median remaining lifespan by 109% over wild-type controls and enhances several health parameters. Importantly, we observed a significant improvement in frailty scores indicating that we were able to improve the healthspan along with increasing the lifespan. Furthermore, in human keratinocytes expressing exogenous OSK, we observed significant epigenetic markers of age-reversal, suggesting a potential reregulation of genetic networks to a younger, potentially healthier state. Together, these results may have important implications for the development of partial reprogramming interventions to reverse age-associated diseases in the elderly.
