Lifeboat Foundation: Safeguarding Humanity
Toggle light / dark theme

Blog

Category: neuroscience – Page 737

Genomic Editing of Non-Coding RNA Genes with CRISPR/Cas9 Ushers in a Potential Novel Approach to Study and Treat Schizophrenia

Circa 2017

Schizophrenia is a genetically related mental illness, in which the majority of genetic alterations occur in the non-coding regions of the human genome. In the past decade, a growing number of regulatory non-coding RNAs (ncRNAs) including microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) have been identified to be strongly associated with schizophrenia. However, the studies of these ncRNAs in the pathophysiology of schizophrenia and the reverting of their genetic defects in restoration of the normal phenotype have been hampered by insufficient technology to manipulate these ncRNA genes effectively as well as a lack of appropriate animal models. Most recently, a revolutionary gene editing technology known as Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated nuclease 9 (Cas9; CRISPR/Cas9) has been developed that enable researchers to overcome these challenges. In this review article, we mainly focus on the schizophrenia-related ncRNAs and the use of CRISPR/Cas9-mediated editing on the non-coding regions of the genomic DNA in proving causal relationship between the genetic defects and the pathophysiology of schizophrenia. We subsequently discuss the potential of translating this advanced technology into a clinical therapy for schizophrenia, although the CRISPR/Cas9 technology is currently still in its infancy and immature to put into use in the treatment of diseases. Furthermore, we suggest strategies to accelerate the pace from the bench to the bedside. This review describes the application of the powerful and feasible CRISPR/Cas9 technology to manipulate schizophrenia-associated ncRNA genes. This technology could help researchers tackle this complex health problem and perhaps other genetically related mental disorders due to the overlapping genetic alterations of schizophrenia with other mental illnesses.

Keywords: CRISPR/Cas9; gene editing; lncRNAs; miRNAs; non-coding RNAs; schizophrenia.

The Brain Has a Built-in System to Keep Unwanted Memories Out, Study Finds

A new study in the Journal of Neuroscience has some answers. By scanning the brains of 24 people actively suppressing a particular memory, the team found a neural circuit that detects, inhibits, and eventually erodes intrusive memories.

A trio of brain structures makes up this alarm system. At the heart is the dACC (for “dorsal anterior cingulate cortex”), a scarf-like structure that wraps around deeper brain regions near the forehead. It acts like an intelligence agency: it monitors neural circuits for intrusive memories, and upon discovery, alerts the “executive” region of the brain. The executive then sends out an abort signal to the brain’s memory center, the hippocampus. Like an emergency stop button, this stops the hippocampus from retrieving the memory.

The entire process happens below our consciousness, suppressing unwanted memories so that they never surface to awareness.

Surprising Discovery: How a Gene Mutation Causes Higher Intelligence in Humans

When genes mutate, it can result in severe diseases of the human nervous system. Neuroscientists at Leipzig University and the University of Würzburg have now used fruit flies to demonstrate how, apart from the negative effect, the mutation of a neuronal gene can have a positive effect – namely higher IQ in humans. They have published their findings in the prestigious journal Brain.

Synapses are the contact points in the brain via which nerve cells ‘talk’ to one another. Disruptions in this communication lead to nervous system diseases, since altered synaptic proteins, for example, can impair this complex molecular mechanism. This can cause mild symptoms, but also very severe disabilities in those affected.

The interest of the two neurobiologists Professor Tobias Langenhan and Professor Manfred Heckmann, from Leipzig and Würzburg respectively, was aroused when they read in a scientific publication about a mutation that damages a synaptic protein. At first, the affected patients attracted scientists’ attention because the mutation caused them to go blind. However, doctors then noticed that the patients were also of above-average intelligence. “It’s very rare for a mutation to lead to improvement rather than loss of function,” says Langenhan, professor and holder of a chair at the Rudolf Schönheimer Institute of Biochemistry at the Faculty of Medicine.

Scientists Discover Master Gene In Mice That Could Restore Hearing Lost In Aging

Hearing loss is normally permanent as sensory cells responsible for transmitting frequency information from the world around us to the brain get damaged from excessive noise and lifestyle factors as we age. Up until now, it’s been challenging to selectively regrow these sensory cells that play an important part in transmitting sound through the outer and inner ear to the brain, but that might be about to change.

In a study involving mice, scientists from Northwestern University have identified a single master gene that can program ear hair cells (known as cochlear hair cells) into becoming either outer or inner ear hair cells required for hearing. The breakthrough is reported in the journal Nature.

“Our finding gives us the first clear cell switch to make one type versus the other,” said lead study author Jaime García-Añoveros, PhD, in a statement. “It will provide a previously unavailable tool to make an inner or outer hair cell. We have overcome a major hurdle.”

Researchers find new function performed by almost half of brain cells

* Astrocytes play a variety of roles with neurons, but until now, scientists did not know that these cells carry electrical impulses.

* Applying new technology, Tufts University scientists recently discovered in mice that astrocytes are electrically active like neurons. Astrocytes play a variety of roles with neurons, but until now, scientists did not know that these cells carry electrical impulses.

Neurotransmitters are chemical messengers that facilitate the transfer of electrical signals between neurons and support the blood-brain barrier. Scientists have long understood that astrocytes control these substances to support neuronal health.

This study breaks ground in showing that neurons release potassium ions, which change the astrocytes’ electrical activity. This modulation affects how the astrocytes control neurotransmitters.

Until now, scientists could not image potassium activity in the brain.

Does Earth Have a Mind and Agency of Its Own?

Does our planet have a mind and agency of its own? This is one of the main questions philosopher and mystic Oberon Zell illuminates in his latest masterpiece GaeaGenesis: Conception and Birth of the Living Earth. Just as we don’t see a bacterium with a naked eye, we don’t quite seem to have an innate ability to perceive the Gaian mind with a “naked” brain. As Dr. Ralph Metzner, Professor Emeritus of California Institute of Integral Studies and Founder-President of The Green Earth Foundation, writes: “Oberon Zell was the first person to conceive and publish the biological and metaphysical foundations of what has become known as the ‘Gaia Theory’ — the unified body and emergent soul of the living Earth… For over 50 years Oberon has been writing and lecturing on Gaian consciousness, and it is high time that he put it all together into a book!” And, indeed, he did.

The newly-released book takes the idea beyond the metaphorical realm postulated by James Lovelock in his “Gaia Hypothesis” and posits that the entire evolution of life on Earth is the literal embryology of a single vast living being — one replicating continuum of DNA and protoplasm. This distinction has significant implications for the subject of this book: The proposition that Mother Earth is a living, sentient being with a “soul” that humans can perceive if they are aware enough to sense it. In essence, the living beings that populate the Earth are cells within a greater macro-organism.

Here’s one of the revelatory passages from the book: To better understand the planet as a living system, we need to go beyond the time scales of human life to the planet’s own time scale, vastly greater than our own. Looked at in this way, the rhythm of day and night might be the pulse of the planet, one full cycle of every hundred thousand human heartbeats. Speeding up time appropriately, we would see the atmosphere and ocean currents swirling round the planet, circulating nutrients and carrying away waste products, much as the blood circulates nutrients and carries away waste in our own bodies.

Longevity Research is an Economic Necessity

It is vital to recognize the immediate economic importance of i nvesting in longevity and healthy-aging sciences.

Aging itself is a complex series of at least 300 biological processes involving more than 10% of our genetic makeup. It follows that methods to combat these effects must be a combination of sciences, from biotech to biophysics and pharmaceuticals. There is no single “silver bullet” solution.

Aging, along with the physical and mental decay that accompanies it, is still widely regarded as a natural and inevitable thing. It is not, it is a degenerative disease in which the physical integrity and structure of our cells decay each time they divide to replace old ones or as part of any healing process.

/* */