Lifeboat Foundation

Glutamate decarboxylase 67-kDa isoform (GAD67), which is encoded by the GAD1 gene, is one of the key enzymes that produce GABA. The reduced expression of GAD67 has been linked to the pathophysiology of schizophrenia. Additionally, the excitatory glutamatergic system plays an important role in the development of this disorder. Animal model studies have revealed that chronic blockade of NMDA-type glutamate receptors can cause GABAergic dysfunction and long-lasting behavioral abnormalities. Based on these findings, we speculated that Gad1 haplodeficiency combined with chronic NMDA receptor blockade would lead to larger behavioral consequences relevant to schizophrenia in a rat model. In this study, we administered an NMDAR antagonist, MK-801 (0.2 mg/kg), to CRISPR/Cas9-generated Gad1^+/− rats during adolescence to test this hypothesis. The MK-801 treated Gad1^+/− rats showed a shorter duration in each rearing episode in the open field test than the saline-treated Gad1^+/+ rats. In contrast, immobility in the forced swim test was increased and fear extinction was impaired in Gad1^+/− rats irrespective of MK-801 treatment. Interestingly, the time spent in the center region of the elevated plus-maze was significantly affected only in the saline-treated Gad1^+/− rats. Additionally, the MK-801-induced impairment of the social novelty preference was not observed in Gad1^+/− rats. These results suggest that the synergistic and additive effects of Gad1 haplodeficiency and NMDA receptor blockade during adolescence on the pathogenesis of schizophrenia may be more limited than expected. Findings from this study also imply that these two factors mainly affect negative or affective symptoms, rather than positive symptoms.

γ-Aminobutyric acid (GABA) is a primary inhibitory neurotransmitter in the central nervous system (Obata, 2013). Post-mortem brain studies on schizophrenia have shown that GABAergic disturbances are part of the pathophysiology of the disorder (Lewis and Sweet, 2009). In particular, the expression level of the GABA-synthesizing enzyme glutamate decarboxylase 67-kDa isoform (GAD67) is lower in the cerebral cortex of patients with schizophrenia than in that of healthy subjects (Guidotti et al., 2000; Volk et al., 2000; Hashimoto et al., 2003; Hashimoto et al., 2008; Curley et al., 2011). GAD67 is encoded by the GAD1 gene, whose SNPs are also suggested to be risk factors for schizophrenia (Addington et al., 2005). We previously reported that Gad1^−/− rats displayed some schizophrenia-relevant behaviors, including working memory, which is important for the functional outcome of schizophrenia (Fujihara et al., 2020a).

Scientists said this full picture of the genome will give humanity a greater understanding of our evolution and biology while also opening the door to medical discoveries in areas like aging, neurodegenerative conditions, cancer and heart disease.

“We’re just broadening our opportunities to understand human disease,” said Karen Miga, an author of one of the six studies published Thursday.

The research caps off decades of work. The first draft of the human genome was announced in a White House ceremony in 2000 by leaders of two competing entities: an international publicly funded project led by an agency of the U.S. National Institutes of Health and a private company, Maryland-based Celera Genomics.

Despite a wealth of knowledge gained in the past three decades concerning the molecular underpinnings of Alzheimer’s disease (AD), progress towards obtaining effective, disease modifying therapies has proven to be challenging. In this manner, numerous clinical trials targeting the production, aggregation, and toxicity of beta-amyloid, have failed to meet efficacy standards. This puts into question the beta-amyloid hypothesis and suggests that additional treatment strategies should be explored. The recent emergence of CRISPR/Cas9 gene editing as a relatively straightforward, inexpensive, and precise system has led to an increased interest of applying this technique in AD. CRISPR/Cas9 gene editing can be used as a direct treatment approach or to help establish better animal models that more faithfully mimic human neurodegenerative diseases. In this manner, this technique has already shown promise in other neurological disorders, such as Huntington’s disease. The purpose of this review is to examine the potential utility of CRISPR/Cas9 as a treatment option for AD by targeting specific genes including those that cause early-onset AD, as well as those that are significant risk factors for late-onset AD such as the apolipoprotein E4 (APOE4) gene.

Keywords: Alzheimer’s disease, CRISPR/Cas9, Gene editing, Treatment, Huntington’s disease, iPSC neurons.

Alzheimer’s Disease (AD) is a progressive and fatal neurodegenerative disorder that primarily affects older adults and is the most common cause of dementia [1]. Currently it afflicts 5.5 million Americans and that number is expected to triple by 2050. At the present time, it is the third leading cause of death behind heart disease and cancer, with an estimated 700,000 Americans ages65 years will have AD when they die [2]. In addition, the cost of the disease is substantial with $259 billion health care dollars going to manage the disease currently, and by the middle of the century costs are predicted to soar over $1.2 trillion, which will completely bankrupt the healthcare system in the USA [3]. Worldwide, 47 million people live with dementia and that number is projected to increase to more than 131 million by 2050 with an estimated worldwide cost of US $818 billion [4].

𝐍𝐞𝐰 𝐀𝐭𝐥𝐚𝐬:

The Neuro-Network.

𝐄𝐧𝐳𝐲𝐦𝐞 𝐛𝐥𝐨𝐜𝐤𝐞𝐫 𝐜𝐨𝐮𝐥𝐝 𝐨𝐩𝐞𝐧 𝐧𝐞𝐰 𝐭𝐫𝐞𝐚𝐭𝐦𝐞𝐧𝐭𝐬 𝐟𝐨𝐫 𝐧𝐞𝐮𝐫𝐨𝐝𝐞𝐠𝐞𝐧𝐞𝐫𝐚𝐭𝐢𝐯𝐞 𝐝𝐢𝐬𝐞𝐚𝐬𝐞𝐬

Continue reading “Enzyme blocker could open new treatments for neurodegenerative diseases” »

A new study led by the Massachusetts Institute of Technology has found that a group of neurons in the brain’s striatum encodes information about the potential outcomes of different decisions. The study was published in the journal, ‘Nature Communications’.

A group of neurons in the brain become particularly active when a behaviour leads to a different outcome than what was expected, which the researchers believed helped the brain adapt to changing circumstances. “A lot of this brain activity deals with surprising outcomes because if an outcome is expected, there’s really nothing to be learned. What we see is that there’s a strong encoding of both unexpected rewards and unexpected negative outcomes,” said Bernard Bloem, a former MIT postdoc and one of the lead authors of the new study.

Impairments in this kind of decision-making are a hallmark of many neuropsychiatric disorders, especially anxiety and depression. The new findings suggested that slight disturbances in the activity of these striatal neurons could swing the brain into making impulsive decisions or becoming paralyzed with indecision, the researchers said. The striatum, located deep within the brain, is known to play a key role in making decisions that require evaluating the outcomes of a particular action. In this study, the researchers wanted to learn more about the neural basis of how the brain makes cost-benefit decisions, in which a behaviour can have a mixture of positive and negative outcomes.

Boston medical researchers in a new groundbreaking study have discovered a “vicious cycle” between daytime napping and Alzheimer’s dementia.

The Brigham and Women’s Hospital researchers found a link between the two: Excessive daytime napping predicted an increased future risk of Alzheimer’s dementia, and a diagnosis of Alzheimer’s dementia sped up the increase in daytime napping during aging.

Daytime napping is common among older adults, but researchers have not known the relationship between daytime napping and cognitive aging.

The study could provide hope for people who have locked-in syndrome. But the study author’s track record is mired with controversy.

Combining AI and robotics technology, researchers have identified new cellular characteristics of Parkinson’s disease in skin cell samples from patients.

#ai #parkinsons #neuroscience #science #robotics

Summary: Combining AI and robotics technology, researchers have identified new cellular characteristics of Parkinson’s disease in skin cell samples from patients.

Continue reading “Artificial Intelligence and Robotics Uncover Hidden Signatures of Parkinson’s Disease” »

AI and neuroscience have too many similarities. While Artificial neural networks mimic the human brain, the human behaviour holds generous data for Artificial intelligence to train itself into human mind.