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Distant recurrence (DR) represents a distinct and lethal trajectory in glioblastoma (GBM), characterized by the colonization of spatially isolated neural niches. The mechanisms by which disseminated tumor cells remodel the microenvironment to overcome anatomical and immunological barriers remain poorly understood. This study aimed to decode the immune ecosystem of DR and elucidate the intercellular crosstalk driving this lethal dissemination.

We performed single-cell RNA sequencing (scRNA-seq) on paired primary and DR tumor specimens from GBM patients, alongside an independent local recurrence (LR) control cohort, to map dynamic cellular shifts. Bioinformatic analyses, including differential abundance testing (Milo), cell-cell communication inference (CellChat), and copy number variation (CNV) analysis, were integrated with clinical co-immunoprecipitation (Co-IP) validation, bidirectional in vitro co-culture assays, and an orthotopic xenograft mouse model.

Our high-resolution atlas revealed a profound remodeling of the microenvironment in DR lesions, characterized by a specific expansion of anti-inflammatory monocyte-derived tumor-associated macrophages (Anti_infl-TAM) and a paradoxical co-enrichment of CD8 + T cells and Tregs. Mechanistically, an OPC-like subpopulation of PTN+ tumor cells acted as a “paracrine architect,” promoting Anti_infl-TAM polarization through the interaction between PTN and NCL. Co-IP assays confirmed direct physical binding of these two proteins in human DR tissues. Functional and bidirectional knockdown assays demonstrated that decoupling this cellular communication, achieved by targeting either PTN in tumor cells or NCL in macrophages, significantly attenuated glioma cell proliferation, migration, invasion, and macrophage polarization. In vivo, silencing PTN markedly reduced Anti_infl-TAM infiltration, suppressed intracranial tumor burden, and prolonged overall survival in tumor-bearing mice.

Dr Martin Wessendorf is a neuroscientist and an associate professor emeritus at the University of Minnesota. His work focuses on how the brainstem controls the function of neurons in the spinal cord. Daniel Wang also researches the brain, with his focus on cognitive neuroscience and neurodegenerative diseases.

They are the authors of a new Frontiers in Neuroanatomy article that explores several brain circuits in four-limbed animals that are cross-wired (meaning one side of the brain connects with the opposite side of the body). It proposes a novel hypothesis about how they might have evolved.

For those who have studied neuroanatomy, you might have wondered about the brain’s cross-wiring and its evolutionary history. We’ll go over different pathways and then propose our hypothesis on why most of these contralateral (midline-crossing) pathways evolved.