Structured illumination microscopy (SIM) is the most preferable system for live-cell super-resolution imaging. It enables the observation of intricate subcellular dynamics. However, conventional SIM has long relied on the complex rotation of one-dimensional stripe illumination at three angles, requiring nine exposures to reconstruct a uniform super-resolution image. This greatly hinders imaging speed and causes unnecessary photobleaching, limiting the available information flux in live-cell imaging.

Professor Xi Peng’s team from the College of Future Technology at Peking University has developed a triangle-beam interference SIM (3I-SIM) that enables gentler, sustained super-resolution live-cell imaging. This novel method upgrades the super-resolution imaging to an unprecedented kilo-Hz speed and half-day-long duration, enabling the study of complex and rapid biological processes with higher data throughput.

The work is published in Nature Photonics.