Researchers at the University of Virginia have created the first comprehensive protein-level atlas of brain development, providing unprecedented insight into how the brain forms and potential implications for understanding neurological disorders. The study, published in Nature Neuroscience, analyzed over 24 million individual cells from mouse brains, revealing detailed molecular pathways that guide brain development from early embryonic stages through early postnatal development.

The research team, led by Professors Christopher Deppmann and Eli Zunder, used an innovative technique called mass cytometry to track 40 different proteins across various brain regions and developmental stages. The approach provided a more detailed view of cellular function than previous studies that primarily examined RNA.

“While RNA studies have given us important insights, proteins are the actual workforce of cells,” explained Deppmann, a professor in the College and Graduate School of Arts & Sciences’ Department of Biology. “By studying proteins directly, we can better understand how cells are functioning and communicating during brain development.”