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Jul 17, 2022

The LightCycler® 480 real-time PCR system: a versatile platform for genetic variation research

Posted by in category: genetics

Circa 2008


From endpoint fluorescence to melting curve analysis.

Today’s research on somatic, genetic and epigenetic variation in eukaryotic cells requires fast, accurate and cost-effective methods for screening large numbers of samples or loci in parallel. Variations identified by genomic sequencing or array studies need to be subsequently confirmed and validated.

Real-time PCR has become a well established technology for this purpose. The plate-based LightCycler 480 system offers a broad selection of methods and applications (Fig. 1).

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