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Oct 2, 2021

Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells

Posted by in category: biotech/medical

In vitro digestion of genomic DNA with Cas9 and single guide RNAs (sgRNAs) yields genome-wide off-target sites at frequencies below 0.1%. Off-target sites can be further reduced with modified sgRNAs.

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