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A novel coronavirus (COVID-19 virus) outbreak has caused a global pandemic resulting in tens of thousands of infections and thousands of deaths worldwide. The RNA-dependent RNA polymerase (RdRp, also named nsp12) is the central component of coronaviral replication/transcription machinery and appears to be a primary target for the antiviral drug, remdesivir. We report the cryo-EM structure of COVID-19 virus full-length nsp12 in complex with cofactors nsp7 and nsp8 at 2.9-Å resolution. In addition to the conserved architecture of the polymerase core of the viral polymerase family, nsp12 possesses a newly identified β-hairpin domain at its N terminus. A comparative analysis model shows how remdesivir binds to this polymerase. The structure provides a basis for the design of new antiviral therapeutics targeting viral RdRp.

Corona Virus Disease 2019 (COVID-19) caused by a novel coronavirus emerged in December 2019 (1–3) and has since become a global pandemic. COVID-19 virus is reported to be a new member of the betacoronavirus genus and is closely related to severe acute respiratory syndrome coronavirus (SARS-CoV) and to several bat coronaviruses (4). Compared to SARS-CoV and MERS-CoV, COVID-19 virus exhibits faster human-to-human transmission, thus leading to the WHO declaration of a world-wide public health emergency (1, 2).

CoVs employ a multi-subunit replication/transcription machinery. A set of non-structural proteins (nsp) produced as cleavage products of the ORF1a and ORF1ab viral polyproteins (5) assemble to facilitate viral replication and transcription. A key component, the RNA-dependent RNA polymerase (RdRp, also known as nsp12), catalyzes the synthesis of viral RNA and thus plays a central role in the replication and transcription cycle of COVID-19 virus, possibly with the assistance of nsp7 and nsp8 as co-factors (6). Nsp12 is therefore considered a primary target for nucleotide analog antiviral inhibitors such as remdesivir, which shows potential for the treatment of COVID-19 viral infections (7, 8). To inform drug design we have determined the structure of nsp12, in complex with its cofactors nsp7 and nsp8 by cryo-Electron Microscopy (Cryo-EM) using two different protocols, one in the absence of DTT (Dataset-1) and the other in the presence of DTT (Dataset-2).

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A woman in her forties has become the latest known case of SARS-CoV-2 infection associated with a “sudden and complete” loss of smell. Known as anosmia, loss of smell is regarded as one of the tell-tale signs of COVID-19.

When an odor enters the nose, neurons in the nasal cavity send messages to the olfactory bulb, a structure in the front part of the brain. These signals are then shipped off to different parts of the brain, leading some medical experts to wonder whether the novel coronavirus could cause adverse neurological reactions.

But how could a virus associated with respiratory infections impact the brain? Researchers are trying to find out. Dr Serena Spudich, a neurologist at the Yale School of Medicine specializing in infectious diseases, says that it is too early to say definitively whether SARS-CoV-2 directly infects the brain but limited data suggests it is a possibility.

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Synthetic microparticles more intricate than some of the most complicated ones found in nature have been produced by a University of Michigan-led international team. They also investigated how that intricacy arises and devised a way to measure it.

The findings pave the way for more stable fluid-and-particle mixes, such as paints, and new ways to twist light—a prerequisite for holographic projectors.

The particles are composed of twisted spikes arranged into a ball a few microns, or millionths of a meter, across.

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A 29-year-old coronavirus patient has improved from serious to serious, but stable condition after receiving multiple doses of plasma from a donor who recovered from coronavirus.

A 29-year-old haredi (ultra-Orthodox) coronavirus patient who is being treated at Samson Assuta Ashdod University Hospital has improved from serious to serious but stable condition, after receiving multiple doses of plasma over the weekend from a donor who recovered from coronavirus, a spokesperson for the hospital told The Jerusalem Post.

On Friday, “with the assistance of Health Minister Ya’acov Litzman and his assistant, a suitable donor, a resident of Jerusalem, was found,” explained MDA director-general Eli Bin.

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Some hospitals have reported unusually high death rates for coronavirus patients on ventilators, and some doctors worry that the machines could be harming certain patients. When people talk of the safety of medicines, they need to also talk of the safety of ventilators as the wrong treatment can kill a patient, and we have enough evidence that ventilators are not working.

NEW YORK (AP) — As health officials around the world push to get more ventilators to treat coronavirus patients, some doctors are moving away from using the breathing machines when they can.

The reason: Some hospitals have reported unusually high death rates for coronavirus patients on ventilators, and some doctors worry that the machines could be harming certain patients.

The evolving treatments highlight the fact that doctors are still learning the best way to manage a virus that emerged only months ago. They are relying on anecdotal, real-time data amid a crush of patients and shortages of basic supplies.

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Rebreather diving has gained popularity over the last few years. As you can imagine, these high tech dive units are not cheap. As with any large investment, buying your first rebreather can be daunting. Before you make the big purchase, it’s crucial that you dive and test out a variety of different models. But where do you start? How do you know what’s the best rebreather for your style of diving?

Though rebreathers all perform the same task: removing additional CO2 from the breathing loop while adding the necessary amount of oxygen or nitrox – The way different models execute this task varies. Remember, a rebreather is a huge investment, and thus it’s important that you take the time to find the right machine for you and your type of diving.

One of our goals here at The Adventure Junkies is to make your life easier when it comes to gear shopping. In this guide, we’ll walk you through some things to consider before buying a rebreather and show you our selection of some of the best models out there.

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