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Mar 30, 2023

Transplantation of clinical-grade human neural stem cells reduces neuroinflammation, prolongs survival and delays disease progression in the SOD1 rats

Posted by in categories: biotech/medical, cryonics, life extension, neuroscience

The hNSCs used in the study have been produced and characterised in the Cell Factory and Biobank of Santa Maria Hospital (Terni, Italy), authorised by the Italian Medicine Agency (AIFA) for the production of hNSCs to be used for clinical trials (aM 54/2018). The methodology applied to isolate, expand, characterise and cryopreserve the lines is based on the Neurosphere Assay26,41,54, and has been used for the production of the cells utilised in phase I trials for Amyotrophic Lateral Sclerosis patients (NCT0164006723) and for Secondary Progressive Multiple Sclerosis patients (NCT03282760, ongoing).

The entire production process, starting from tissue procurement to cryopreservation is compliant to cGMP guidelines and approved by AIFA. The hNSCs are obtained from foetal brain tissue derived from fetuses that underwent miscarriage or natural in utero death upon receiving the signed informed consent from the mother. Forty-eight hours prior to implantation, hNSCs were plated in the growth medium at a concentration of 10,000 cells/cm2. On the day of surgery, hNSCs were collected by centrifugation, viable cells were counted by Trypan blue exclusion criteria, and the correct number of cells were re-suspended in HBSS for the transplant.

SOD1 transgenic male rats were randomly divided into three experimental groups: (i) transplanted with hNSCs (hNSC rats, n = 15); (ii) treated with HBSS (HBSS rats, n = 15) and (iii) untreated (CTRL rats, n = 22). An additional group of non-transgenic littermates (wild-type, WT, n = 9) were used as controls for symptomatic evaluation of the colony. Tacrolimus (FK506) and cyclosporine (cyclosporin A) are the principal immunosuppressive drugs that have been applied for solid organ transplantation55,56 and have been translated to stem cell treatments for PD57 and ALS22. In animal models, despite differences in potency, both drugs showed excellent survival rates for grafts across many comparative studies58,59. Our previous results44,45 showed that hNSCs can survive—without signs of rejection—in the rat brain up to 6 months under transient immunosuppression treatment, with cyclosporin A. On the bases of these results, we applied the same immunosuppressive treatment with administration of cyclosporine A (15 mg/kg/day subcutaneous; Sandimmne, Novartis) that was initiated on the day of transplantation and continued for 15 days after surgery (for all animal groups).

Mar 30, 2023

Quantum on a Microgram Scale

Posted by in categories: particle physics, quantum physics

An experiment with an acoustic resonator demonstrates the quantum superposition of atoms—nearly matching the ability of matter interferometers to test quantumness on macroscopic scales.

Mar 30, 2023

We’re nowhere near reaching the maximum human life span, controversial study suggests

Posted by in category: life extension

Human longevity records may be broken in the next few decades, a new modeling study suggests.

Mar 30, 2023

Research confirms a big cause of Alzheimer’s disease

Posted by in categories: biotech/medical, neuroscience

Alzheimer’s disease is a very common illness that affects older people worldwide, and it is one of the main causes of dementia. Researchers have been working for more than twenty years to find out what causes Alzheimer’s, but they have not yet discovered the exact reason, and there is no cure for the disease.

Mar 30, 2023

College Students Are About to Put a Robot on the Moon Before NASA

Posted by in categories: robotics/AI, space travel

A commercial spaceflight in May will take a Carnegie Mellon-designed rover, named Iris, to the lunar surface.

Mar 30, 2023

How does unprocessed, minimally-processed, and ultra-processed food impact dietary quality?

Posted by in categories: food, health

In a recent study published in the Journal of the Academy of Nutrition and Dietetics, researchers assessed the impact of consuming unprocessed, minimally processed (UMP), and ultra-processed foods (UPFs) on diet quality.

Study: Intakes of unprocessed and minimally processed and ultra-processed food are associated with diet-quality in female and male health professionals in the United States: a prospective analysis.Image Credit: Parilov/Shutterstock.com

Mar 30, 2023

Green macroalgae Ulva: Future superfood?

Posted by in categories: chemistry, futurism

A recent study published in the journal Algal Research summarized the existing knowledge on bioactive compounds in green seaweeds and Ulva spp., focusing on its application as a future superfood.

Seaweeds are macroalgae, colonizing brackish water bodies and seas, and are classified into brown, green, and red algae. Research suggests that seaweeds are enriched with bioactive compounds with therapeutic potential. Seaweeds are also good sources of nutrients, antioxidants, and dietary fiber and have a low caloric value.

Ulva lactuca, a green alga, is a source of carotenoids, ulvan (a polysaccharide), proteins, minerals, vitamin C, and dietary fibers. In the present study, the authors discussed the chemistry and applications of bioactive compounds of green seaweeds, mainly focusing on U. lactuca and emphasizing its application as a superfood.

Mar 30, 2023

The Open Letter on AI Doesn’t Go Far Enough

Posted by in categories: ethics, robotics/AI

The real move at play here, by so called AI Ethics clowns, is a complete shut down of Ai, and AI research. That IS their end goal — end game. See if can really turn it off 6 months. ha! Ok, how about 2 more years! etc… etc…

Ya publicly tipped your hand.


An open letter published today calls for “all AI labs to immediately pause for at least 6 months the training of AI systems more powerful than GPT-4.”

Continue reading “The Open Letter on AI Doesn’t Go Far Enough” »

Mar 30, 2023

New study discovers how to reverse hearing loss

Posted by in category: futurism

Baby mice can regenerate damaged hair cells — and now that we know how they do it, maybe we can, too in order to reverse hearing loss.

Mar 29, 2023

Proteomic Analysis of Huntington’s Disease Medium Spiny Neurons Identifies Alterations in Lipid Droplets

Posted by in categories: biotech/medical, neuroscience

Huntington’s disease (HD) is a neurodegenerative disease caused by a CAG repeat expansion in the Huntingtin (HTT) gene. The resulting polyglutamine (polyQ) tract alters the function of the HTT protein. Although HTT is expressed in different tissues, the medium spiny projection neurons (MSNs) in the striatum are particularly vulnerable in HD. Thus, we sought to define the proteome of human HD patient–derived MSNs. We differentiated HD72 induced pluripotent stem cells and isogenic controls into MSNs and carried out quantitative proteomic analysis. Using data-dependent acquisitions with FAIMS for label-free quantification on the Orbitrap Lumos mass spectrometer, we identified 6,323 proteins with at least two unique peptides. Of these, 901 proteins were altered significantly more in the HD72-MSNs than in isogenic controls. Functional enrichment analysis of upregulated proteins demonstrated extracellular matrix and DNA signaling (DNA replication pathway, double-strand break repair, G1/S transition) with the highest significance. Conversely, processes associated with the downregulated proteins included neurogenesis-axogenesis, the brain-derived neurotrophic factor-signaling pathway, Ephrin-A: EphA pathway, regulation of synaptic plasticity, triglyceride homeostasis cholesterol, plasmid lipoprotein particle immune response, interferon-γ signaling, immune system major histocompatibility complex, lipid metabolism and cellular response to stimulus. Moreover, proteins involved in the formation and maintenance of axons, dendrites, and synapses (e.g., Septin protein members) were dysregulated in HD72-MSNs. Importantly, lipid metabolism pathways were altered, and using quantitative image, we found analysis that lipid droplets accumulated in the HD72-MSN, suggesting a deficit in the turnover of lipids possibly through lipophagy. Our proteomics analysis of HD72-MSNs identified relevant pathways that are altered in MSNs and confirm current and new therapeutic targets for HD.

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