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Adeno-associated virus (AAV) has emerged as a leading platform for gene therapy, enabling the delivery of therapeutic DNA to target cells. However, the potential of AAV to deliver protein payloads has been unexplored. In this study, we engineered a protein carrier AAV (pcAAV) to package and deliver proteins by inserting binding domains on the interior capsid surface. These binding domains mediate the packaging of specific target proteins through interaction with cognate peptides or protein tags during the capsid assembly process. We demonstrate the packaging of multiple proteins, including green fluorescent protein, Streptococcus pyogenes Cas9, Cre recombinase, and the engineered peroxidase APEX2. Packaging efficiency is modulated by the binding domain insertion site, the viral protein isoform containing the binding domain, and the subcellular localization of the target protein. We show that pcAAV can enter cells and deliver the protein payload and that enzymes retain their activity after packaging. Importantly, this protein packaging capability can be translated to multiple AAV serotypes. Our work establishes AAV as a protein delivery vehicle, significantly expanding the utility of this viral vector for biomedical applications.

Desalination can offer a relief in probably trillions of dollars of savings for countries even when drought comes.


Water desalination plants could replace expensive chemicals with new carbon cloth electrodes that remove boron from seawater, an important step of turning seawater into safe drinking water.

A study describing the new technology has been published in Nature Water by engineers at the University of Michigan and Rice University.

Many plans have been hatched to bring more water to CA, but it’s better to build desalination plants. And even better to power them with small nuclear reactors. Thirty desal plants produces a billion gallons/day and would cost the same as a water pipeline stealing water from the Pacific Northwest.

Disney has finally taken us back to The Grid. This video goes over everything revealed in the official Tron Ares Trailer as well as other information we’ve learned.

Thumbnail Credit: https://gmunk.com/TRON-Concepts.
Background Credit: https://youtu.be/cragUPBty8U
3.0 Concept Art Credit: https://www.reddit.com/r/tron/comments/1hnir4i/tron_30_end_o…and_pitch/
Ares Concept Art Credit: https://www.instagram.com/p/DIMc2uXvJhQ/?hl=en&img_index=1

Join The Tron Community Discord Server & Discuss Tron Ares! https://discord.gg/QvGrBKrtGW

Sacred waters with remarkable healing powers. Ancient mystics who lived for hundreds of years. A scientific breakthrough may provide the answers to eternal life. Throughout history many have searched for the secret to everlasting life, but so far, it’s eluded them. But is it possible to cheat death and live forever? See more in Season 3, Episode 5, \.

Scientists have discovered a ‘concerning’ secret in the unknown depths of a 410 foot Great Blue Hole.

The mysterious sinkhole is located just off the coast of Belize where experts have managed to extract a sample of material from the bottom.

And now the sediment core they retrieved is revealing secrets from the past six millennia.