To enable prospective lineage tracing with chromatin accessibility capture, we have developed ‘CellTag-multi’. CellTag-multi is based on our previous CellTagging technology, which uses sequential lentiviral delivery of CellTags (heritable random barcodes) to enable the construction of multilevel lineage trees^7,16. Here we introduce a strategy in which CellTags, expressed as polyadenylated transcripts, can be captured in both scRNA-seq and scATAC-seq assays allowing for independent tracking of clonal transcriptional and epigenomic state.

We validate this method using in vitro hematopoiesis, a well-characterized model of multilineage differentiation, and demonstrate highly accurate reconstruction of lineage relationships and capture of lineage-specific progenitor cell states across scRNA-seq and scATAC-seq. Moreover, the addition of chromatin accessibility information to gene expression allows for an improvement in the prediction of differentiation outcome from early progenitor state. We also deploy CellTag-multi in the direct lineage reprogramming of fibroblasts to iEPs, to characterize early gene regulatory changes in rare subpopulations of cells that successfully reprogram. This application reveals how chromatin is remodeled following the expression of reprogramming TFs, enabling deeper insight into gene regulatory network reconfiguration. We uncover the TF Foxd2 as a facilitator of on-target reprogramming, increasing the efficiency of MEF to iEP conversion.

Startup Light Bio has created a bioluminescent petunia using mushroom genes and plans to start shipping the plants next spring.

‘There should not be one ounce of trust left that any advertiser has in Google Ads,’ according to Greg Finn.

ALPHA-g is first to observe antihydrogen in freefall.

Scientists have captured the fleeting moment when a heart starts beating, a feat made all the more remarkable when you consider the relative brevity of a zebrafish’s life.

In a tight window about 20 hours into zebrafish development, the embryos’ developing hearts jumped into action, emerging as one from an ensemble of single cells.

“It was like somebody had flipped on a switch,” says Harvard University biophysicist Adam Cohen, senior author of the new study, which imaged zebrafish embryos snug in custom-made agarose molds to capture this once-in-a-lifetime event.

New analysis of trilobite fossil gives clues about ancient creature’s feeding behavior.

Believe it or not, air contains as much water as six times the world’s rivers combined.

This is mostly in the form of water vapor, and it actually replenishes naturally every 8 to 10 days — give or take. This offers a valuable potential reservoir of freshwater that could be tapped relatively easily.

All we need is the right kind of technology.

Scientists believe that Homo sapiens, or modern humans, evolved into our present form approximately 300,000 years ago.