Chlorogenic acid (CGA) and taurine are well-known antioxidant compounds reported to reduce skin cellular senescence. However, the biological mechanisms underlying their skin-protective effects remain unclear.

Methods:

In this study, we conducted transcriptome-wide RNA sequencing to profile gene expression changes in human epidermal keratinocytes, melanocytes, and fibroblasts following treatment with CGA, taurine, or their combination. To identify aging-related genes, we integrated evidence from aging databases, perceived-age GWAS, enrichment in aging-related gene ontology and pathways, and drug-gene interaction annotations. Validation of representative genes was performed using quantitative real-time PCR.