Unlock the full potential of CRISPR technology while ensuring precision and safety! In this video, we dive deep into the science of CRISPR gene editing, explore the challenges of off-target effects, and reveal cutting-edge strategies to minimize risks.
📌 Key Topics Covered:

1️⃣ What is CRISPR?

Discover the origins of CRISPR-Cas9, its revolutionary impact on genetics, agriculture, and medicine, and the latest advancements like base editing and AI-driven optimization.
2️⃣ Understanding Off-Target Effects.

Learn why unintended DNA modifications occur, how gRNA promiscuity and nuclease activity contribute to risks, and proven mitigation strategies (e.g., HiFi Cas9, dual gRNA systems).
3️⃣ Off-Target Prediction & Detection.

Explore bioinformatics tools (e.g., CRISOT) and advanced detection methods like Whole Genome Sequencing (WGS), LAM-HTGTS, and Digenome-seq for unbiased, high-sensitivity analysis.
4️⃣ Validation & Solutions.

See how CD Genomics’ off-target validation service combines multiplex PCR, Illumina sequencing, and cloud-based analytics to deliver publication-ready results with unmatched accuracy.