{"id":99910,"date":"2019-12-18T18:46:39","date_gmt":"2019-12-19T02:46:39","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2019\/12\/first-images-of-an-upgraded-crispr-tool"},"modified":"2019-12-18T18:46:39","modified_gmt":"2019-12-19T02:46:39","slug":"first-images-of-an-upgraded-crispr-tool","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2019\/12\/first-images-of-an-upgraded-crispr-tool","title":{"rendered":"First images of an \u2018upgraded\u2019 CRISPR tool"},"content":{"rendered":"<p><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/first-images-of-an-upgraded-crispr-tool2.jpg\"><\/a><\/p>\n<p>Columbia scientists have captured the first images of a new gene editing tool that could improve upon existing CRISPR-based tools. The team developed the tool, called INTEGRATE, after discovering a unique \u201cjumping gene\u201d in Vibrio cholerae bacteria that could insert large genetic payloads in the genome without introducing DNA breaks.<\/p>\n<p>In the new study, published today in <i>Nature<\/i>, the researchers harnessed a Nobel Prize-winning technique called cryo-electron microscopy to freeze the gene editing complex in action, revealing high-resolution details about how it works.<\/p>\n<p>\u201cWe showed in our first study how to leverage INTEGRATE for targeted DNA insertions in <a href=\"https:\/\/phys.org\/tags\/bacterial+cells\/\" rel=\"tag\" class=\"\">bacterial cells<\/a>,\u201d says Sam Sternberg, Ph.D., assistant professor of biochemistry &amp; molecular biophysics at Columbia University Vagelos College of Physicians and Surgeons, who led the research with Israel Fernandez, Ph.D., assistant professor of biochemistry &amp; <a href=\"https:\/\/phys.org\/tags\/molecular+biophysics\/\" rel=\"tag\" class=\"\">molecular biophysics<\/a> at Columbia. \u201cThese new images, a wonderful collaboration with Israel Fern\u00e1ndez\u2019s lab, explain the biology with incredible molecular detail and will help us improve the system by guiding protein engineering efforts.\u201d<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Columbia scientists have captured the first images of a new gene editing tool that could improve upon existing CRISPR-based tools. The team developed the tool, called INTEGRATE, after discovering a unique \u201cjumping gene\u201d in Vibrio cholerae bacteria that could insert large genetic payloads in the genome without introducing DNA breaks. In the new study, published [\u2026]<\/p>\n","protected":false},"author":511,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1902,11,412],"tags":[],"class_list":["post-99910","post","type-post","status-publish","format-standard","hentry","category-bioengineering","category-biotech-medical","category-genetics"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/99910","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/511"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=99910"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/99910\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=99910"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=99910"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=99910"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}