{"id":90045,"date":"2019-04-28T11:22:42","date_gmt":"2019-04-28T18:22:42","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2019\/04\/crispr-accuracy-increased-50-fold"},"modified":"2019-04-28T11:22:42","modified_gmt":"2019-04-28T18:22:42","slug":"crispr-accuracy-increased-50-fold","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2019\/04\/crispr-accuracy-increased-50-fold","title":{"rendered":"CRISPR accuracy increased 50-fold"},"content":{"rendered":"<p><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/crispr-accuracy-increased-50-fold.jpg\"><\/a><\/p>\n<p><strong>Biomedical engineers at Duke University, North Carolina, have developed a method for improving the accuracy of CRISPR genome editing by an average of 50-fold. They believe it can be easily translated to any of the technology\u2019s continually expanding formats.<\/strong><\/p>\n<p>The approach adds a short tail to the guide RNA which is used to identify a sequence of DNA for editing. This added tail folds back and binds onto itself, creating a \u201clock\u201d that can only be undone by the targeted DNA sequence.<\/p>\n<p>\u201cCRISPR is generally incredibly accurate, but there are examples that have shown off-target activity, so there\u2019s been broad interest across the field in increasing specificity,\u201d said Charles Gersbach, Professor of Biomedical Engineering at Duke. \u201cBut the solutions proposed thus far cannot be easily translated between different CRISPR systems.\u201d<\/p>\n<p><a href=\"https:\/\/www.futuretimeline.net\/blog\/2019\/04\/28.htm\" target=\"_blank\" rel=\"noopener noreferrer\"><\/p>\n<div style=\"clear:both;\">Read more<\/div>\n<p><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Biomedical engineers at Duke University, North Carolina, have developed a method for improving the accuracy of CRISPR genome editing by an average of 50-fold. They believe it can be easily translated to any of the technology\u2019s continually expanding formats. The approach adds a short tail to the guide RNA which is used to identify a [\u2026]<\/p>\n","protected":false},"author":396,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[11,38],"tags":[],"class_list":["post-90045","post","type-post","status-publish","format-standard","hentry","category-biotech-medical","category-engineering"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/90045","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/396"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=90045"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/90045\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=90045"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=90045"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=90045"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}