{"id":90023,"date":"2019-04-27T17:02:29","date_gmt":"2019-04-28T00:02:29","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2019\/04\/a-deceptively-simple-tweak-to-crispr-makes-it-50-times-more-accurate"},"modified":"2019-04-27T17:02:29","modified_gmt":"2019-04-28T00:02:29","slug":"a-deceptively-simple-tweak-to-crispr-makes-it-50-times-more-accurate","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2019\/04\/a-deceptively-simple-tweak-to-crispr-makes-it-50-times-more-accurate","title":{"rendered":"A Deceptively Simple Tweak to CRISPR Makes It 50 Times More Accurate"},"content":{"rendered":"<p><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/a-deceptively-simple-tweak-to-crispr-makes-it-50-times-more-accurate2.jpg\"><\/a><\/p>\n<p>Without ensuring high levels of accuracy, any proposed CRISPR gene therapy becomes a genetic crapshoot.<\/p>\n<p>Now, a team from Duke University may have found a universal workaround\u2014a trick to fundamentally boost CRISPR\u2019s accuracy in almost all its forms. <a href=\"https:\/\/www.nature.com\/articles\/s41587-019-0095-1\">Published this month in <em>Nature Biotechnology<\/em><\/a>, the team\u2019s study tweaked the design of guide RNAs, the indispensable targeting \u201cblood hound\u201d of the CRISPR duo that hunts down specific DNA sequences before its partner Cas makes the cut.<\/p>\n<p>The upgrade is deceptively simple: tag a \u201clocking\u201d structure to one end of the guide RNA so that only the targeted DNA can unleash the power of the Cas scissors. Yet <em>exactly<\/em> because the tweak is so easy, guide RNA 2.0 can fundamentally tune the accuracy of multiple CRISPR systems\u2014not just those relying on the <a href=\"https:\/\/singularityhub.com\/2016\/01\/21\/crisprcas9-genome-editing-is-a-huge-deal-but-its-just-the-tip-of-the-iceberg\/\">classic Cas9<\/a>, but also newer diagnostic systems that deploy <a href=\"https:\/\/singularityhub.com\/2018\/05\/02\/crispr-on-a-chip-for-diagnosing-cancer-may-soon-be-a-thing\/\">Cas12a<\/a> and other flavors\u2014by as much as 200-fold.<\/p>\n<p><a href=\"https:\/\/singularityhub.com\/2019\/04\/27\/a-deceptively-simple-tweak-to-crispr-makes-it-50-times-more-accurate\/\" target=\"_blank\" rel=\"noopener noreferrer\"><\/p>\n<div style=\"clear:both;\">Read more<\/div>\n<p><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Without ensuring high levels of accuracy, any proposed CRISPR gene therapy becomes a genetic crapshoot. Now, a team from Duke University may have found a universal workaround\u2014a trick to fundamentally boost CRISPR\u2019s accuracy in almost all its forms. Published this month in Nature Biotechnology, the team\u2019s study tweaked the design of guide RNAs, the indispensable [\u2026]<\/p>\n","protected":false},"author":396,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[11,412],"tags":[],"class_list":["post-90023","post","type-post","status-publish","format-standard","hentry","category-biotech-medical","category-genetics"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/90023","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/396"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=90023"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/90023\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=90023"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=90023"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=90023"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}