{"id":32529,"date":"2016-12-10T12:34:31","date_gmt":"2016-12-10T20:34:31","guid":{"rendered":"http:\/\/lifeboat.com\/blog\/2016\/12\/gene-therapy-prevents-cancer-cells-from-surviving-in-hypoxic-environment"},"modified":"2017-06-04T13:59:04","modified_gmt":"2017-06-04T20:59:04","slug":"gene-therapy-prevents-cancer-cells-from-surviving-in-hypoxic-environment","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2016\/12\/gene-therapy-prevents-cancer-cells-from-surviving-in-hypoxic-environment","title":{"rendered":"Gene Therapy Prevents Cancer Cells from Surviving in Hypoxic Environment"},"content":{"rendered":"<p><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/gene-therapy-prevents-cancer-cells-from-surviving-in-hypoxic-environment.jpg\"><\/a><\/p>\n<p>Gene therapy techniques were used to insert a peptide into cultures of human cancer cells that blocked their ability to use the enzyme Hypoxia-inducible factor-1, a heterodimeric transcription factor that enables cell survival under low oxygen conditions by altering the transcription of over 300 genes.<\/p>\n<p>Hypoxia inducible factor-1 (HIF-1) is a heterodimeric transcription factor that acts as the master regulator of cellular response to reduced oxygen levels, thus playing a key role in the adaptation, survival, and progression of tumors. There is significant evidence that inhibition of HIF-1 would be beneficial for cancer therapy, since tumor cells must thrive in a microenvironment characterized by lack of oxygen. In previous work, investigators at the University of Southampton (United Kingdom) discovered a cyclic hexapeptide (cyclo-CLLFVY) that inhibited the HIF-1alpha\/HIF-1beta protein\u2013protein interaction in vitro and prevented HIF-1-mediated hypoxia-response signaling in cells. This cyclic peptide was identified by screening a library that contained more than 3.2 million compounds.<\/p>\n<p>With a view to demonstrating the potential for encoding the production of a therapeutic agent in response to a disease marker, the investigators engineered human cells with an additional chromosomal control circuit that conditionally encoded the production of the cyclic peptide HIF-1 inhibitor. They then demonstrated the conditional production of the HIF-1 inhibitor in response to hypoxia, and its inhibitory effect on HIF-1 dimerization and downstream hypoxia-response signaling.<\/p>\n<p><!-- Link: <a href=\"http:\/\/www.biotechdaily.com\/genomics-proteomics\/articles\/294767165\/gene-therapy-prevents-cancer-cells-from-surviving-in-hypoxic-environment.html\">http:\/\/www.biotechdaily.com\/genomics-proteomics\/articles\/294...nment.html<\/a> --><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Gene therapy techniques were used to insert a peptide into cultures of human cancer cells that blocked their ability to use the enzyme Hypoxia-inducible factor-1, a heterodimeric transcription factor that enables cell survival under low oxygen conditions by altering the transcription of over 300 genes. Hypoxia inducible factor-1 (HIF-1) is a heterodimeric transcription factor that [\u2026]<\/p>\n","protected":false},"author":395,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[11],"tags":[],"class_list":["post-32529","post","type-post","status-publish","format-standard","hentry","category-biotech-medical"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/32529","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/395"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=32529"}],"version-history":[{"count":3,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/32529\/revisions"}],"predecessor-version":[{"id":65617,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/32529\/revisions\/65617"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=32529"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=32529"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=32529"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}