{"id":243991,"date":"2026-09-13T01:17:46","date_gmt":"2026-09-13T06:17:46","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2026\/09\/hdac10-promotes-th17-differentiation-and-il17adriven-neutrophilic-airway-inflammation-in-severe-asthma"},"modified":"2026-09-13T01:17:46","modified_gmt":"2026-09-13T06:17:46","slug":"hdac10-promotes-th17-differentiation-and-il17adriven-neutrophilic-airway-inflammation-in-severe-asthma","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2026\/09\/hdac10-promotes-th17-differentiation-and-il17adriven-neutrophilic-airway-inflammation-in-severe-asthma","title":{"rendered":"HDAC10 promotes Th17 differentiation and IL17Adriven neutrophilic airway inflammation in severe asthma"},"content":{"rendered":"

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To delineate HDAC10\u2019s function in T cells, we engineered CD4+<\/sup> T cell-specific Hdac10<\/i> knockout (KO) mice (Hdac10<\/i>fl\/fl<\/i><\/sup>-CD4Cre<\/i><\/i>) by crossing Hdac10<\/i>fl\/fl<\/i><\/sup> mice with CD4Cre<\/i> mice (Figure S2 <\/a>A), which were genotyped using PCR (Figure S2 <\/a>B). The deletion of HDAC10 in CD4+<\/sup> T cells was confirmed by western blotting (WB) and RT-qPCR (Figures S2 <\/a>C and S2D). We observed that Hdac10<\/i> absence in CD4+<\/sup> T cells had no effects on T cell development and differentiation in thymus (Figure S2 <\/a>E and S2F) and T cells composition, apoptosis, or proliferation in spleen and mesenteric lymph nodes (mLN) (Figures S2 <\/a>G\u2013S2R) at steady state. Collectively, these data demonstrate that HDAC10 is dispensable for steady-state T cell development.<\/p>\n

To determine HDAC10\u2019s role in CD4+<\/sup> T cells during allergic airway inflammation, we employed a neutrophilic asthma mouse model. Hdac10<\/i>fl\/fl<\/sup>-CD4Cre<\/i><\/i> asthmatic mice exhibited lowered airway resistance compared with Hdac10<\/i>fl\/fl<\/sup> asthmatic mice (Figure 2A). Total cell counts and the number of neutrophils in BALF were profoundly reduced in Hdac10<\/i>fl\/fl<\/sup>-CD4Cre<\/i><\/i> asthmatic mice compared with the littermates (Figures 2B and 2C). Hematoxylin and eosin (HE) staining confirmed attenuated inflammation score in Hdac10<\/i>fl\/fl<\/sup>-CD4Cre<\/i><\/i> asthmatic mice (Figures 2D and 2E). Peribronchial trichrome (Masson) and periodic acid Schiff (PAS) staining showed a reduction in collagen deposition and mucus production in Hdac10<\/i>fl\/fl<\/sup>-CD4Cre<\/i><\/i> asthmatic mice (Figures 2F\u20132I). Furthermore, IHC for myeloperoxidase (MPO) showed reduced neutrophil infiltration in lung tissues of Hdac10<\/i>fl\/fl<\/sup>-CD4Cre<\/i><\/i> asthmatic mice (Figures 2J and 2K).<\/p>\n","protected":false},"excerpt":{"rendered":"

To delineate HDAC10\u2019s function in T cells, we engineered CD4+ T cell-specific Hdac10 knockout (KO) mice (Hdac10fl\/fl-CD4Cre) by crossing Hdac10fl\/fl mice with CD4Cre mice (Figure S2 A), which were genotyped using PCR (Figure S2 B). The deletion of HDAC10 in CD4+ T cells was confirmed by western blotting (WB) and RT-qPCR (Figures S2 C and [\u2026]<\/p>\n","protected":false},"author":662,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"class_list":["post-243991","post","type-post","status-publish","format-standard","hentry","category-futurism"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/243991","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/662"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=243991"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/243991\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=243991"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=243991"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=243991"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}