{"id":242313,"date":"2026-08-06T17:12:41","date_gmt":"2026-08-06T22:12:41","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2026\/08\/pediatric-ependymoma-in-the-molecular-era-realworld-experience-with-diagnostic-correlation-and-longterm-clinical-outcomes-neurooncology"},"modified":"2026-08-06T17:12:41","modified_gmt":"2026-08-06T22:12:41","slug":"pediatric-ependymoma-in-the-molecular-era-realworld-experience-with-diagnostic-correlation-and-longterm-clinical-outcomes-neurooncology","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2026\/08\/pediatric-ependymoma-in-the-molecular-era-realworld-experience-with-diagnostic-correlation-and-longterm-clinical-outcomes-neurooncology","title":{"rendered":"Pediatric ependymoma in the molecular era: realworld experience with diagnostic correlation and longterm clinical outcomes NeuroOncology"},"content":{"rendered":"<p><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/pediatric-ependymoma-in-the-molecular-era-realworld-experience-with-diagnostic-correlation-and-longterm-clinical-outcomes-neurooncology2.jpg\"><\/a><\/p>\n<p>Real-world longitudinal data documenting the diagnostic impact of DNA methylation array (MA) profiling in pediatric ependymoma remain sparse. We report a single-center retrospective analysis evaluating MA-driven reclassification, molecular subgroup distribution, and long-term survival outcomes in a national pediatric referral cohort.<\/p>\n<p>Sixty-three pediatric patients with a histological ependymoma diagnosis treated at Motol and Homolka University Hospital (2010\u20132025) were included. DNA methylation profiling, RNA sequencing, copy number variation and t-SNE analysis were performed. Survival was estimated by the Kaplan\u2013Meier method.<\/p>\n<p>MA confirmed ependymoma in 48 patients (76.2%) and reclassified 15 (23.8%) as non-ependymoma entities, including newly described tumor types. The reclassification rate was 36.1% in the pre-2019 cohort versus 7.4% post-2019. Posterior fossa group A (PFA) ependymoma was the predominant subgroup (<i>n<\/i> = 26). Ten-year overall survival (OS) was 69.6% and event-free survival (EFS) 47.7%. Gross total resection was the only factor significantly associated with improved survival (OS 75% vs. 40%, <i>p<\/i> = 0.017). Chromosome 1q gain, identified exclusively in PFA patients, was associated with a high relapse rate despite standard therapy.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Real-world longitudinal data documenting the diagnostic impact of DNA methylation array (MA) profiling in pediatric ependymoma remain sparse. We report a single-center retrospective analysis evaluating MA-driven reclassification, molecular subgroup distribution, and long-term survival outcomes in a national pediatric referral cohort. Sixty-three pediatric patients with a histological ependymoma diagnosis treated at Motol and Homolka University Hospital [\u2026]<\/p>\n","protected":false},"author":662,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[11],"tags":[],"class_list":["post-242313","post","type-post","status-publish","format-standard","hentry","category-biotech-medical"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/242313","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/662"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=242313"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/242313\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=242313"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=242313"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=242313"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}