{"id":241820,"date":"2026-07-30T00:14:37","date_gmt":"2026-07-30T05:14:37","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2026\/07\/stable-dna-building-blocks-enable-faster-oligonucleotide-synthesis-without-oxidation"},"modified":"2026-07-30T00:14:37","modified_gmt":"2026-07-30T05:14:37","slug":"stable-dna-building-blocks-enable-faster-oligonucleotide-synthesis-without-oxidation","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2026\/07\/stable-dna-building-blocks-enable-faster-oligonucleotide-synthesis-without-oxidation","title":{"rendered":"Stable DNA building blocks enable faster oligonucleotide synthesis without oxidation"},"content":{"rendered":"<p><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/stable-dna-building-blocks-enable-faster-oligonucleotide-synthesis-without-oxidation.jpg\"><\/a><\/p>\n<p>The chemical synthesis of oligonucleotides (ONs) is central to modern molecular biology, diagnostics and nucleic acid therapeutics. While demand for high-quality ONs is increasing, the conventional synthetic method has long-standing efficiency challenges. Widely adopted P(III)-phosphoramidite-based ON synthesis requires an oxidation step after every nucleotide coupling cycle and uses moisture-sensitive building blocks, adding complexity to the workflow and slowing the process.<\/p>\n<p>Early studies of ON synthesis showed that pentavalent phosphorus [P(V)] chemistry could form linkages between nucleotides. However, practical limitations, including unstable intermediates, slow coupling, harsh deprotection or poor performance during chain elongation, prevented these methods from replacing P(III)-based phosphoramidite chemistry.<\/p>\n<p>A recent study led by Associate Professor Noriko Saito-Tarashima of the Graduate School of Pharmaceutical Sciences at Tokushima University in Japan, along with Nana Mihara, a doctoral student from the same institution, investigated whether nucleoside 3\u2032-phosphorofluoridates [P(V)\u2013F] could be used as stable building blocks for ON synthesis without requiring a separate oxidation step.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The chemical synthesis of oligonucleotides (ONs) is central to modern molecular biology, diagnostics and nucleic acid therapeutics. While demand for high-quality ONs is increasing, the conventional synthetic method has long-standing efficiency challenges. Widely adopted P(III)-phosphoramidite-based ON synthesis requires an oxidation step after every nucleotide coupling cycle and uses moisture-sensitive building blocks, adding complexity to the [\u2026]<\/p>\n","protected":false},"author":662,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[11,19],"tags":[],"class_list":["post-241820","post","type-post","status-publish","format-standard","hentry","category-biotech-medical","category-chemistry"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/241820","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/662"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=241820"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/241820\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=241820"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=241820"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=241820"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}