{"id":233368,"date":"2026-03-15T22:19:44","date_gmt":"2026-03-16T03:19:44","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2026\/03\/a-single-cell-transcriptional-reference-for-the-functional-and-developmental-diversity-of-neonatal-innate-lymphoid-cells"},"modified":"2026-03-15T22:19:44","modified_gmt":"2026-03-16T03:19:44","slug":"a-single-cell-transcriptional-reference-for-the-functional-and-developmental-diversity-of-neonatal-innate-lymphoid-cells","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2026\/03\/a-single-cell-transcriptional-reference-for-the-functional-and-developmental-diversity-of-neonatal-innate-lymphoid-cells","title":{"rendered":"A single-cell transcriptional reference for the functional and developmental diversity of neonatal innate lymphoid cells"},"content":{"rendered":"<p style=\"padding-right: 20px\"><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/a-single-cell-transcriptional-reference-for-the-functional-and-developmental-diversity-of-neonatal-innate-lymphoid-cells2.jpg\"><\/a><\/p>\n<p>Mononuclear cells (MNCs) were isolated from fresh umbilical cord blood (CB) via a Ficoll gradient (<i>n<\/i> = 3). Unwanted cells were depleted using biotinylated antibodies (anti-CD3, anti-CD14, anti-CD19, and CD66b) and magnetic beads. The cells were stained to faithfully sort cILC1s (Lin<sup>\u2212<\/sup>CD94<sup>\u2212<\/sup>CD127<sup>+<\/sup>CD117<sup>\u2212<\/sup>CRTH2<sup>\u2212<\/sup>), cILC2s (Lin<sup>\u2212<\/sup>CD94<sup>\u2212<\/sup>CD127<sup>+<\/sup>CD117<sup>\u2212\/+ <\/sup>CRTH2<sup>+<\/sup>), cILC3s (Lin<sup>\u2212<\/sup>CD94<sup>\u2212<\/sup>CD127<sup>+<\/sup>CD117<sup>+<\/sup>CRTH2<sup>\u2212<\/sup>), and NK cells (Lin<sup>\u2212<\/sup>CD94<sup>+<\/sup>), as previously described.<a id=\"crosref1290\" href=\"http:\/\/dlvr.it\/TRVfHr#bib5\" data-xml-rid=\"bib5\"><sup>5<\/sup><\/a><sup>,<\/sup><a id=\"crosref1295\" href=\"http:\/\/dlvr.it\/TRVfHr#bib7\" data-xml-rid=\"bib7\"><sup>7<\/sup><\/a><sup>,<\/sup><a id=\"crosref1300\" href=\"http:\/\/dlvr.it\/TRVfHr#bib33\" data-xml-rid=\"bib33\"><sup>33<\/sup><\/a> The four individual populations were individually multiplexed, pooled, and stained with Ab-Seq antibodies.<\/p>\n<p>(A) scRNA-seq was performed via the BD Rhapsody protocol.<\/p>\n<p>(B and C) UMAP visualization of sorted populations (B) and individual clusters of CB cILCs and NK cells with color coding of the individual cluster 0\u201310. The following clusters were identified: 0, CD56<sup>dim<\/sup> NK cells (<i>GZMB<\/i><sup><i>high<\/i><\/sup>); 1, CD56<sup>dim<\/sup> NK cells (<i>GZMB<\/i><sup><i>low<\/i><\/sup>); 2, cILC2s (<i>GATA3<\/i>); 3, cILC progenitor (<i>KIT<\/i>); 4, CD56<sup>bright<\/sup> NK cells (<i>GZMK<\/i>); 5, intermediate zone (NK\/cILCs); 6, cILC1s (<i>CD5<\/i>); 7, activated CD56<sup>dim<\/sup> NK cells (<i>PCNA<\/i>); 8, cycling CD56<sup>dim<\/sup> NK cells (<i>MKI67<\/i>); 9, cILC3s (<i>RORC<\/i>); and 10, CD56<sup>dim<\/sup> NK cells (<i>FOXP2<\/i>) \u00a9.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Mononuclear cells (MNCs) were isolated from fresh umbilical cord blood (CB) via a Ficoll gradient (n = 3). Unwanted cells were depleted using biotinylated antibodies (anti-CD3, anti-CD14, anti-CD19, and CD66b) and magnetic beads. The cells were stained to faithfully sort cILC1s (Lin\u2212CD94\u2212CD127+CD117\u2212CRTH2\u2212), cILC2s (Lin\u2212CD94\u2212CD127+CD117\u2212\/+ CRTH2+), cILC3s (Lin\u2212CD94\u2212CD127+CD117+CRTH2\u2212), and NK cells (Lin\u2212CD94+), as previously described.5,7,33 The [\u2026]<\/p>\n","protected":false},"author":662,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[11],"tags":[],"class_list":["post-233368","post","type-post","status-publish","format-standard","hentry","category-biotech-medical"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/233368","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/662"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=233368"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/233368\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=233368"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=233368"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=233368"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}