{"id":232999,"date":"2026-03-10T23:24:15","date_gmt":"2026-03-11T04:24:15","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2026\/03\/highly-efficient-expression-of-dna-peptide-conjugates-in-growth-arrested-cells"},"modified":"2026-03-10T23:24:15","modified_gmt":"2026-03-11T04:24:15","slug":"highly-efficient-expression-of-dna-peptide-conjugates-in-growth-arrested-cells","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2026\/03\/highly-efficient-expression-of-dna-peptide-conjugates-in-growth-arrested-cells","title":{"rendered":"Highly efficient expression of DNA-peptide conjugates in growth-arrested cells"},"content":{"rendered":"<p><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/highly-efficient-expression-of-dna-peptide-conjugates-in-growth-arrested-cells2.jpg\"><\/a><\/p>\n<p>Mohamedshah <i>et al.<\/i> present an enzymatic strategy for covalently linking nuclear localization sequence (NLS) peptides to DNA cassettes by incorporating a strained cyclooctyne and using SPAAC chemistry, greatly enhancing transfection efficiency compared to previous methods. [ <a href=\"https:\/\/www.nature.com\/articles\/s41467-025-68167-5\">https:\/\/www.nature.com\/articles\/s41467-025-68167-5<\/a>](<a href=\"https:\/\/www.nature.com\/articles\/s41467-025-68167-5\">https:\/\/www.nature.com\/articles\/s41467-025-68167-5<\/a>) <\/p>\n<hr>\n<p>Efficient nuclear delivery of DNA remains a major challenge in non-viral gene therapy. Here the authors present an improved workflow for generating DNA oligonucleotide-peptide conjugates which are ligated to linear DNA and achieve nuclear localization.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Mohamedshah et al. present an enzymatic strategy for covalently linking nuclear localization sequence (NLS) peptides to DNA cassettes by incorporating a strained cyclooctyne and using SPAAC chemistry, greatly enhancing transfection efficiency compared to previous methods. [ https:\/\/www.nature.com\/articles\/s41467-025-68167-5](https:\/\/www.nature.com\/articles\/s41467-025-68167-5) Efficient nuclear delivery of DNA remains a major challenge in non-viral gene therapy. Here the authors present an [\u2026]<\/p>\n","protected":false},"author":636,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[11,19],"tags":[],"class_list":["post-232999","post","type-post","status-publish","format-standard","hentry","category-biotech-medical","category-chemistry"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/232999","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/636"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=232999"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/232999\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=232999"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=232999"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=232999"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}