{"id":227237,"date":"2025-12-16T21:02:28","date_gmt":"2025-12-17T03:02:28","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2025\/12\/a-chemically-inducible-leakless-cre-recombinase-by-split-protein-based-efficient-and-enhanced-degradation-speed"},"modified":"2025-12-16T21:02:28","modified_gmt":"2025-12-17T03:02:28","slug":"a-chemically-inducible-leakless-cre-recombinase-by-split-protein-based-efficient-and-enhanced-degradation-speed","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2025\/12\/a-chemically-inducible-leakless-cre-recombinase-by-split-protein-based-efficient-and-enhanced-degradation-speed","title":{"rendered":"A chemically inducible, leakless Cre recombinase by split-protein-based efficient and enhanced degradation (SPEED)"},"content":{"rendered":"<p><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/a-chemically-inducible-leakless-cre-recombinase-by-split-protein-based-efficient-and-enhanced-degradation-speed.jpg\"><\/a><\/p>\n<p>A combination of self-assembling split-Cre with a destabilizing domain system is found to be an effective way to improve the degradation efficiency of destabilized Cre in the absence of Trimethoprim (Tmp) while maintaining efficient TMP-inducible DNA recombination.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>A combination of self-assembling split-Cre with a destabilizing domain system is found to be an effective way to improve the degradation efficiency of destabilized Cre in the absence of Trimethoprim (Tmp) while maintaining efficient TMP-inducible DNA recombination.<\/p>\n","protected":false},"author":511,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[11,19],"tags":[],"class_list":["post-227237","post","type-post","status-publish","format-standard","hentry","category-biotech-medical","category-chemistry"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/227237","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/511"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=227237"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/227237\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=227237"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=227237"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=227237"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}