{"id":224516,"date":"2025-11-05T01:09:14","date_gmt":"2025-11-05T07:09:14","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2025\/11\/adapting-next-generation-sequencing-to-in-process-crispr-cas9-genome-editing-of-recombinant-acmnpv-vectors-from-shotgun-to-tiled-amplicon-sequencing"},"modified":"2025-11-05T01:09:14","modified_gmt":"2025-11-05T07:09:14","slug":"adapting-next-generation-sequencing-to-in-process-crispr-cas9-genome-editing-of-recombinant-acmnpv-vectors-from-shotgun-to-tiled-amplicon-sequencing","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2025\/11\/adapting-next-generation-sequencing-to-in-process-crispr-cas9-genome-editing-of-recombinant-acmnpv-vectors-from-shotgun-to-tiled-amplicon-sequencing","title":{"rendered":"Adapting Next-Generation Sequencing to in Process CRISPR-Cas9 Genome Editing of Recombinant AcMNPV Vectors: From Shotgun to Tiled-Amplicon Sequencing"},"content":{"rendered":"<p style=\"padding-right: 20px\"><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/adapting-next-generation-sequencing-to-in-process-crispr-cas9-genome-editing-of-recombinant-acmnpv-vectors-from-shotgun-to-tiled-amplicon-sequencing2.jpg\"><\/a><\/p>\n<p>The alphabaculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is the most commonly used virus in the Baculovirus Expression Vector System (BEVS) and has been utilized for the production of many human and veterinary biologics. AcMNPV has a large dsDNA genome that remains understudied, and relatively unmodified from the wild-type, especially considering how extensively utilized it is as an expression vector. Previously, our group utilized CRISPR-Cas9 genome engineering that revealed phenotypic changes when baculovirus genes are targeted using either co-expressed sgRNA or transfected sgRNA into a stable insect cell line that produced the Cas9 protein.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The alphabaculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is the most commonly used virus in the Baculovirus Expression Vector System (BEVS) and has been utilized for the production of many human and veterinary biologics. AcMNPV has a large dsDNA genome that remains understudied, and relatively unmodified from the wild-type, especially considering how extensively utilized it is [\u2026]<\/p>\n","protected":false},"author":662,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[11,38],"tags":[],"class_list":["post-224516","post","type-post","status-publish","format-standard","hentry","category-biotech-medical","category-engineering"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/224516","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/662"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=224516"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/224516\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=224516"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=224516"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=224516"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}