{"id":224081,"date":"2025-10-26T22:22:47","date_gmt":"2025-10-27T03:22:47","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2025\/10\/mesoscale-volumetric-fluorescence-imaging-at-nanoscale-resolution-by-photochemical-sectioning"},"modified":"2025-10-26T22:22:47","modified_gmt":"2025-10-27T03:22:47","slug":"mesoscale-volumetric-fluorescence-imaging-at-nanoscale-resolution-by-photochemical-sectioning","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2025\/10\/mesoscale-volumetric-fluorescence-imaging-at-nanoscale-resolution-by-photochemical-sectioning","title":{"rendered":"Mesoscale volumetric fluorescence imaging at nanoscale resolution by photochemical sectioning"},"content":{"rendered":"<p><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/mesoscale-volumetric-fluorescence-imaging-at-nanoscale-resolution-by-photochemical-sectioning2.jpg\"><\/a><\/p>\n<p>I first explored this amazing work back when it was a preprint! Wang et al. herein developed VIPS (volumetric imaging via photochemical sectioning), a way of using UV light to remove layers of expanded tissue-hydrogel, allowing combination of high-resolution lattice-light sheet microscopy with expansion microscopy. Link: [ <a href=\"https:\/\/www.science.org\/doi\/10.1126\/science.adr9109\">https:\/\/www.science.org\/doi\/10.1126\/science.adr9109<\/a>]<\/p>\n<p>In my opinion, this technology has enormous future promise for high-throughput connectomics! They will need to improve their labeling density so that higher expansion factors can be used, but this problem is well-studied and I think the issue will likely be solvable with additional resources\/effort.<\/p>\n<hr>\n<p>Optical nanoscopy of intact biological specimens has been transformed by recent advancements in hydrogel-based tissue clearing and expansion, enabling the imaging of cellular and subcellular structures with molecular contrast. However, existing high-resolution fluorescence microscopes are physically limited by objective-to-specimen distance, which prevents the study of whole-mount specimens without physical sectioning. To address this challenge, we developed a photochemical strategy for spatially precise sectioning of specimens. By combining serial photochemical sectioning with lattice light-sheet imaging and petabyte-scale computation, we imaged and reconstructed axons and myelin sheaths across entire mouse olfactory bulbs at nanoscale resolution.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>I first explored this amazing work back when it was a preprint! Wang et al. herein developed VIPS (volumetric imaging via photochemical sectioning), a way of using UV light to remove layers of expanded tissue-hydrogel, allowing combination of high-resolution lattice-light sheet microscopy with expansion microscopy. Link: [ https:\/\/www.science.org\/doi\/10.1126\/science.adr9109] In my opinion, this technology has enormous [\u2026]<\/p>\n","protected":false},"author":636,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3,19,1523,4],"tags":[],"class_list":["post-224081","post","type-post","status-publish","format-standard","hentry","category-biological","category-chemistry","category-computing","category-nanotechnology"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/224081","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/636"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=224081"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/224081\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=224081"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=224081"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=224081"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}