{"id":222393,"date":"2025-09-24T07:04:24","date_gmt":"2025-09-24T12:04:24","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2025\/09\/a-more-precise-crispr-platform-enables-large-scale-gene-screening-in-live-mouse-brains"},"modified":"2025-09-24T07:04:24","modified_gmt":"2025-09-24T12:04:24","slug":"a-more-precise-crispr-platform-enables-large-scale-gene-screening-in-live-mouse-brains","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2025\/09\/a-more-precise-crispr-platform-enables-large-scale-gene-screening-in-live-mouse-brains","title":{"rendered":"A more precise CRISPR platform enables large-scale gene screening in live mouse brains"},"content":{"rendered":"<p><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/a-more-precise-crispr-platform-enables-large-scale-gene-screening-in-live-mouse-brains2.jpg\"><\/a><\/p>\n<p>Over the past few decades, biomedical researchers and neuroscientists have devised increasingly advanced techniques to study and alter neurophysiological processes. These include CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats), a sophisticated tool to edit specific genes in some animals, including mice, rats, zebrafish and fruit flies.<\/p>\n<p>Researchers at University of California, San Francisco led by Martin Kampmann recently introduced a more precise CRISPR screening platform that can be applied directly in living tissue, enabling the screening of a larger number of genes at once. The new technique, called CRISPR screening by AAV episome sequencing (CrAAVe-seq), was introduced in <a href=\"https:\/\/www.nature.com\/articles\/s41593-025-02043-9#citeas\" target=\"_blank\">a paper<\/a> published in Nature Neuroscience.<\/p>\n<p>\u201cHuman cell-based systems are valuable but cannot fully capture the complexity of the brain,\u201d Biswa Ramani, co-first author of the paper, told Medical Xpress. \u201cMice often remain the most effective model for many <a href=\"https:\/\/medicalxpress.com\/tags\/neurological+diseases\/\" rel=\"tag\" class=\"\">neurological diseases<\/a> because their brains preserve the diversity and organization of cell types that cannot be replicated in a dish.\u201d<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Over the past few decades, biomedical researchers and neuroscientists have devised increasingly advanced techniques to study and alter neurophysiological processes. These include CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats), a sophisticated tool to edit specific genes in some animals, including mice, rats, zebrafish and fruit flies. Researchers at University of California, San Francisco led by [\u2026]<\/p>\n","protected":false},"author":732,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[11,47],"tags":[],"class_list":["post-222393","post","type-post","status-publish","format-standard","hentry","category-biotech-medical","category-neuroscience"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/222393","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/732"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=222393"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/222393\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=222393"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=222393"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=222393"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}