{"id":221703,"date":"2025-09-10T23:14:35","date_gmt":"2025-09-11T04:14:35","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2025\/09\/ultra-bright-and-stable-red-and-near-infrared-squaraine-fluorophores-for-in-vivo-two-photon-imaging"},"modified":"2025-09-10T23:14:35","modified_gmt":"2025-09-11T04:14:35","slug":"ultra-bright-and-stable-red-and-near-infrared-squaraine-fluorophores-for-in-vivo-two-photon-imaging","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2025\/09\/ultra-bright-and-stable-red-and-near-infrared-squaraine-fluorophores-for-in-vivo-two-photon-imaging","title":{"rendered":"Ultra-Bright and \u2014Stable Red and Near-Infrared Squaraine Fluorophores for In Vivo Two-Photon Imaging"},"content":{"rendered":"<p style=\"padding-right: 20px\"><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/ultra-bright-and-stable-red-and-near-infrared-squaraine-fluorophores-for-in-vivo-two-photon-imaging2.jpg\"><\/a><\/p>\n<p>Fluorescent dyes that are bright, stable, small, and biocompatible are needed for high-sensitivity two-photon imaging, but the combination of these traits has been elusive. We identified a class of squaraine derivatives with large two-photon action cross-sections (up to 10,000 GM) at near-infrared wavelengths critical for in vivo imaging. We demonstrate the biocompatibility and stability of a red-emitting squaraine-rotaxane (SeTau-647) by imaging dye-filled neurons in vivo over 5 days, and utility for sensitive subcellular imaging by synthesizing a specific peptide-conjugate label for the synaptic protein PSD-95.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Fluorescent dyes that are bright, stable, small, and biocompatible are needed for high-sensitivity two-photon imaging, but the combination of these traits has been elusive. We identified a class of squaraine derivatives with large two-photon action cross-sections (up to 10,000 GM) at near-infrared wavelengths critical for in vivo imaging. We demonstrate the biocompatibility and stability of [\u2026]<\/p>\n","protected":false},"author":636,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[47],"tags":[],"class_list":["post-221703","post","type-post","status-publish","format-standard","hentry","category-neuroscience"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/221703","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/636"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=221703"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/221703\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=221703"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=221703"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=221703"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}