{"id":219057,"date":"2025-07-30T12:13:08","date_gmt":"2025-07-30T17:13:08","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2025\/07\/rna-seq-outperforms-dna-methods-in-detecting-actionable-cancer-mutations"},"modified":"2025-07-30T12:13:08","modified_gmt":"2025-07-30T17:13:08","slug":"rna-seq-outperforms-dna-methods-in-detecting-actionable-cancer-mutations","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2025\/07\/rna-seq-outperforms-dna-methods-in-detecting-actionable-cancer-mutations","title":{"rendered":"RNA-seq outperforms DNA methods in detecting actionable cancer mutations"},"content":{"rendered":"<p><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/rna-seq-outperforms-dna-methods-in-detecting-actionable-cancer-mutations.jpg\"><\/a><\/p>\n<p>Hospital for Sick Children in Toronto researchers are reporting that targeted RNA sequencing can detect clinically actionable alterations in 87% of tumors and provide decisive findings where DNA-seq either fails, returns no variant, or is not informative.<\/p>\n<p>Cancer treatments have seen tremendous improvements in recent years, in part due to highly specific targeting and <a href=\"https:\/\/medicalxpress.com\/tags\/diagnostic+techniques\/\" rel=\"tag\" class=\"\">diagnostic techniques<\/a>.<\/p>\n<p>DNA-based methods dominate molecular cancer diagnostics but struggle to detect <a href=\"https:\/\/medicalxpress.com\/tags\/gene+fusions\/\" rel=\"tag\" class=\"\">gene fusions<\/a> and assess splice site consequences. RNA sequencing enables sensitive fusion detection and direct assessment of transcript-level disruption caused by splicing mutations.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Hospital for Sick Children in Toronto researchers are reporting that targeted RNA sequencing can detect clinically actionable alterations in 87% of tumors and provide decisive findings where DNA-seq either fails, returns no variant, or is not informative. Cancer treatments have seen tremendous improvements in recent years, in part due to highly specific targeting and diagnostic [\u2026]<\/p>\n","protected":false},"author":396,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[11,412],"tags":[],"class_list":["post-219057","post","type-post","status-publish","format-standard","hentry","category-biotech-medical","category-genetics"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/219057","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/396"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=219057"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/219057\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=219057"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=219057"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=219057"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}