{"id":216176,"date":"2025-06-18T09:13:27","date_gmt":"2025-06-18T14:13:27","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2025\/06\/new-method-enables-scientists-to-surpass-the-resolution-limit-of-fluorescence-microscopy"},"modified":"2025-06-18T09:13:27","modified_gmt":"2025-06-18T14:13:27","slug":"new-method-enables-scientists-to-surpass-the-resolution-limit-of-fluorescence-microscopy","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2025\/06\/new-method-enables-scientists-to-surpass-the-resolution-limit-of-fluorescence-microscopy","title":{"rendered":"New method enables scientists to surpass the resolution limit of fluorescence microscopy"},"content":{"rendered":"<p><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/new-method-enables-scientists-to-surpass-the-resolution-limit-of-fluorescence-microscopy3.jpg\"><\/a><\/p>\n<p>Imagine you\u2019re sitting at a pond, listening to the din of croaking frogs. You want to know how many frogs are in the pond, but you can\u2019t pick out the individual croaks\u2014only the combined sound rising and falling in volume as frogs start and stop communicating.<\/p>\n<p>But what if you were able to examine these volume changes to figure out how many frogs are in the pond?<\/p>\n<p>That\u2019s the idea behind a <a href=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acs.nanolett.4c06304\" target=\"_blank\">new method<\/a> developed by the Funke Lab at Janelia to count the individual <a href=\"https:\/\/phys.org\/tags\/molecules\/\" rel=\"tag\" class=\"\">molecules<\/a> contained in a single spot of light detected by a fluorescence microscope\u2014a quantity important for understanding the underlying biology of a living system. The paper is published in the journal Nano Letters.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Imagine you\u2019re sitting at a pond, listening to the din of croaking frogs. You want to know how many frogs are in the pond, but you can\u2019t pick out the individual croaks\u2014only the combined sound rising and falling in volume as frogs start and stop communicating. But what if you were able to examine these [\u2026]<\/p>\n","protected":false},"author":427,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3],"tags":[],"class_list":["post-216176","post","type-post","status-publish","format-standard","hentry","category-biological"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/216176","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/427"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=216176"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/216176\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=216176"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=216176"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=216176"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}