{"id":209685,"date":"2025-03-25T05:11:41","date_gmt":"2025-03-25T10:11:41","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2025\/03\/genetic-diversity-and-molecular-evolution-of-3-carboxymuconate-cyclase-gp60-70-the-major-antigen-in-pathogenic-sporothrix-species"},"modified":"2025-03-25T05:11:41","modified_gmt":"2025-03-25T10:11:41","slug":"genetic-diversity-and-molecular-evolution-of-3-carboxymuconate-cyclase-gp60-70-the-major-antigen-in-pathogenic-sporothrix-species","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2025\/03\/genetic-diversity-and-molecular-evolution-of-3-carboxymuconate-cyclase-gp60-70-the-major-antigen-in-pathogenic-sporothrix-species","title":{"rendered":"Genetic diversity and molecular evolution of 3-carboxymuconate cyclase (Gp60\u201370), the major antigen in pathogenic Sporothrix species"},"content":{"rendered":"<p><a class=\"blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/genetic-diversity-and-molecular-evolution-of-3-carboxymuconate-cyclase-gp60-70-the-major-antigen-in-pathogenic-sporothrix-species.jpg\"><\/a><\/p>\n<p>Sporotrichosis, a neglected tropical disease caused by <i>Sporothrix<\/i> species, is a growing concern, particularly due to the emergence of highly virulent, cat-transmitted <i>S. brasiliensis<\/i>. Rapid diagnosis and surveillance are crucial for controlling sporotrichosis. This study investigated the 3-carboxymuconate cyclase (<i>CMC<\/i>) gene, which encodes the major <i>Sporothrix<\/i> antigen (Gp60\u201370), as a molecular marker to understand the genetic diversity and evolution of these fungi. Analysis of 104 isolates (<i>S. brasiliensis<\/i>, <i>S. <i>schenckii<\/i><\/i>, <i>S. globosa<\/i>, and <i>S. luriei<\/i>) revealed 79 unique haplotypes, demonstrating superior discriminatory power over traditional molecular markers. High\u2013<i>CMC<\/i> polymorphisms, especially in <i>S. brasiliensis<\/i> and <i>S. <i>schenckii<\/i><\/i>, suggest recent population expansion or positive selection, potentially driven by environmental pressures such as polyaromatic hydrocarbon pollutants. The conserved chromosomal location of <i>CMC<\/i> in pathogenic <i>Sporothrix<\/i> and its absence in less virulent species suggest a role in virulence. Identifying conserved residues within predicted B-cell epitopes provides targets for diagnostics and therapeutics. Additionally, we identified <i>N<\/i>-linked glycosylation sequons (e.g. NGS at 62, NNT at 225, and NGT at 373\/374) conserved in pathogenic <i>Sporothrix<\/i> but absent in environmental <i>Sordariomycetes<\/i>, possibly contributing to pathogenicity and niche adaptation. This study establishes <i>CMC<\/i> as a valuable marker for understanding <i>Sporothrix<\/i> evolution and virulence, aiding in sporotrichosis management.<\/p>\n<p>Display full size.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Sporotrichosis, a neglected tropical disease caused by Sporothrix species, is a growing concern, particularly due to the emergence of highly virulent, cat-transmitted S. brasiliensis. Rapid diagnosis and surveillance are crucial for controlling sporotrichosis. This study investigated the 3-carboxymuconate cyclase (CMC) gene, which encodes the major Sporothrix antigen (Gp60\u201370), as a molecular marker to understand the [\u2026]<\/p>\n","protected":false},"author":427,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[11,385,412],"tags":[],"class_list":["post-209685","post","type-post","status-publish","format-standard","hentry","category-biotech-medical","category-evolution","category-genetics"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/209685","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/427"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=209685"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/209685\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=209685"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=209685"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=209685"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}