{"id":208697,"date":"2025-03-14T09:25:55","date_gmt":"2025-03-14T14:25:55","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2025\/03\/crispr-cascade-test-detects-bloodstream-infections-in-minutes-without-amplification"},"modified":"2025-03-14T09:25:55","modified_gmt":"2025-03-14T14:25:55","slug":"crispr-cascade-test-detects-bloodstream-infections-in-minutes-without-amplification","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2025\/03\/crispr-cascade-test-detects-bloodstream-infections-in-minutes-without-amplification","title":{"rendered":"CRISPR-Cascade test detects bloodstream infections in minutes without amplification"},"content":{"rendered":"<p><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/crispr-cascade-test-detects-bloodstream-infections-in-minutes-without-amplification2.jpg\"><\/a><\/p>\n<p>University of Illinois at Urbana-Champaign researchers have developed a CRISPR-based diagnostic tool capable of detecting bloodstream infections in minutes without the need for nucleic acid amplification. The CRISPR-Cascade assay achieves attomolar sensitivity and incorporates an OR-gated logic function to identify multiple pathogens simultaneously through DNA from pathogens associated with bloodstream infections.<\/p>\n<p>Bloodstream infections require rapid identification to prevent complications, yet standard diagnostic methods rely on <a href=\"https:\/\/phys.org\/tags\/polymerase+chain+reaction\/\" rel=\"tag\" class=\"\">polymerase chain reaction<\/a> (PCR) and isothermal amplification techniques that have built-in processing times. CRISPR-based detection tools such as SHERLOCK and DETECTR have improved specificity but continue to depend on amplification, limiting their turnaround time and practicality in clinical settings.<\/p>\n<p>In the study, \u201cAmplification-free, OR-gated CRISPR-Cascade reaction for pathogen detection in blood samples,\u201d <a href=\"https:\/\/pnas.org\/doi\/10.1073\/pnas.2420166122\" target=\"_blank\">published<\/a> in the <i>Proceedings of the National Academy of Sciences<\/i>, researchers conducted a laboratory-based investigation to determine whether a CRISPR-driven feedback loop could detect pathogenic DNA at ultra-low concentrations without amplification.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>University of Illinois at Urbana-Champaign researchers have developed a CRISPR-based diagnostic tool capable of detecting bloodstream infections in minutes without the need for nucleic acid amplification. The CRISPR-Cascade assay achieves attomolar sensitivity and incorporates an OR-gated logic function to identify multiple pathogens simultaneously through DNA from pathogens associated with bloodstream infections. Bloodstream infections require rapid [\u2026]<\/p>\n","protected":false},"author":427,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[11],"tags":[],"class_list":["post-208697","post","type-post","status-publish","format-standard","hentry","category-biotech-medical"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/208697","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/427"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=208697"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/208697\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=208697"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=208697"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=208697"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}