{"id":205646,"date":"2025-02-05T20:04:30","date_gmt":"2025-02-06T02:04:30","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2025\/02\/production-of-novel-theranostic-nano-vector-based-on-superparamagnetic-iron-oxide-nanoparticles-mir-497-targeting-colorectal-cancer"},"modified":"2025-02-05T20:04:30","modified_gmt":"2025-02-06T02:04:30","slug":"production-of-novel-theranostic-nano-vector-based-on-superparamagnetic-iron-oxide-nanoparticles-mir-497-targeting-colorectal-cancer","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2025\/02\/production-of-novel-theranostic-nano-vector-based-on-superparamagnetic-iron-oxide-nanoparticles-mir-497-targeting-colorectal-cancer","title":{"rendered":"Production of novel theranostic nano-vector based on superparamagnetic iron oxide nanoparticles\/miR-497 targeting colorectal cancer"},"content":{"rendered":"<p><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/production-of-novel-theranostic-nano-vector-based-on-superparamagnetic-iron-oxide-nanoparticles-mir-497-targeting-colorectal-cancer.jpg\"><\/a><\/p>\n<p>Colorectal cancer (CRC) is a serious public health concern worldwide. Immune checkpoint inhibition medication is likely to remain a crucial part of CRC clinical management. This study aims to create new super paramagnetic iron oxide nano-carrier (SPION) that can effectively transport miRNA to specific CRC cell lines. In addition, evaluate the efficiency of this nano-formulation as a therapeutic candidate for CRC. Bioinformatics tools were used to select a promising tumor suppressor miRNA (mir-497-5p). Green route, using <i>Fusarium oxyporium<\/i> fungal species, manipulated for the synthesis of SPION@Ag@Cs nanocomposite as a carrier of miR-497-5p. That specifically targets the suppression of PD1\/PDL1 and CTLA4pathways for colorectal therapy. UV\/visible and FTIR spectroscopy, Zeta potential and MTT were used to confirm the allocation of the miR-497 on SPION@Ag@Cs and its cytotoxicity against CRC cell lines. Immunofluorescence was employed to confirm transfection of cells with miR-497@NPs, and the down-regulation of CTLA4 in HT29, and Caco2 cell lines. On the other hand, PDL1 showed a significant increase in colorectal cell lines (HT-29 and Caco-2) in response to mir497-5p@Nano treatment. The data suggest that the mir-497-loaded SPION@Ag@Cs nano-formulation could be a good candidate for the suppression of CTLA4in CRC human cell lines. Consequently, the targeting miR-497\/CTLA4 axis is a potential immunotherapy treatment strategy for CRC.<\/p>\n<hr>\n<p>Elfiky, A.M., Eid, M.M., El-Manawaty, M. <i>et al. <i>Sci Rep<\/i> <b>15<\/b>, 4,247 (2025). <a href=\"https:\/\/doi.org\/10.1038\/s41598-025-88165-3\">https:\/\/doi.org\/10.1038\/s41598-025-88165-3<\/a><\/p>\n<p><a data-test=\"citation-link\" data-track=\"click\" data-track-action=\"download article citation\" data-track-label=\"link\" data-track-external=\"\" rel=\"nofollow\" href=\"https:\/\/citation-needed.springer.com\/v2\/references\/10.1038\/s41598-025-88165-3?format=refman&flavour=citation\">Download citation<\/a>.<\/i><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Colorectal cancer (CRC) is a serious public health concern worldwide. Immune checkpoint inhibition medication is likely to remain a crucial part of CRC clinical management. This study aims to create new super paramagnetic iron oxide nano-carrier (SPION) that can effectively transport miRNA to specific CRC cell lines. In addition, evaluate the efficiency of this nano-formulation [\u2026]<\/p>\n","protected":false},"author":534,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[11,1495,4],"tags":[],"class_list":["post-205646","post","type-post","status-publish","format-standard","hentry","category-biotech-medical","category-health","category-nanotechnology"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/205646","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/534"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=205646"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/205646\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=205646"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=205646"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=205646"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}