{"id":205584,"date":"2025-02-05T06:32:09","date_gmt":"2025-02-05T12:32:09","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2025\/02\/nicotinamide-riboside-supplementation-alleviates-testicular-aging-induced-by-disruption-of-qprt%e2%80%90dependent-nad-de-novo-synthesis-in-mice"},"modified":"2025-02-05T06:32:09","modified_gmt":"2025-02-05T12:32:09","slug":"nicotinamide-riboside-supplementation-alleviates-testicular-aging-induced-by-disruption-of-qprt%e2%80%90dependent-nad-de-novo-synthesis-in-mice","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2025\/02\/nicotinamide-riboside-supplementation-alleviates-testicular-aging-induced-by-disruption-of-qprt%e2%80%90dependent-nad-de-novo-synthesis-in-mice","title":{"rendered":"Nicotinamide Riboside Supplementation Alleviates Testicular Aging Induced by Disruption of Qprt\u2010Dependent NAD+ De Novo Synthesis in Mice"},"content":{"rendered":"<p>In this study, we have demonstrated the crucial role of NAD<sup>+<\/sup> homeostasis, particularly through the de novo synthesis pathway mediated by <i>Qprt<\/i>, in maintaining spermatogenesis with age. The deletion of <i>Qprt<\/i> led to progressive declines in NAD<sup>+<\/sup> levels, particularly after 6 months of age, which were associated with significant defects in germ cell survival and mitochondrial function in spermatocytes. These disruptions manifested as impaired progression through meiosis, defective DNA double-strand break repair, and abnormal meiotic sex chromosome inactivation. Our findings also highlight the therapeutic potential of NAD<sup>+<\/sup> precursor supplementation, as nicotinamide riboside effectively rescued the observed spermatogenic abnormalities in <i>Qprt<\/i>-deficient mice, emphasizing the importance of NAD<sup>+<\/sup> in reproductive health and aging.<\/p>\n<p>NAD<sup>+<\/sup> can be synthesized through three pathways: the Preiss-Handler pathway, the salvage pathway, and the de novo pathway (Liu et al. 2018 ; Harjes 2019). In the de novo pathway, the essential amino acid tryptophan serves as a substrate, with Qprt catalyzing the formation of nicotinic acid mononucleotide, which is subsequently converted into NAD<sup>+<\/sup> via a series of enzymatic reactions in the Preiss-Handler pathway. Coordinated regulation of these three pathways is crucial for maintaining intracellular NAD<sup>+<\/sup> levels, which are essential for cellular function, a decline in NAD<sup>+<\/sup> levels can lead to various pathological and physiological conditions (Minhas et al. 2019 ; Zhang et al. 2019a). In this study, we identified that Qprt, the rate-limiting enzyme in the NAD<sup>+<\/sup> de novo synthesis pathway, is predominantly expressed in spermatocytes within the testes.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>In this study, we have demonstrated the crucial role of NAD+ homeostasis, particularly through the de novo synthesis pathway mediated by Qprt, in maintaining spermatogenesis with age. The deletion of Qprt led to progressive declines in NAD+ levels, particularly after 6 months of age, which were associated with significant defects in germ cell survival and [\u2026]<\/p>\n","protected":false},"author":662,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[11,269,1901],"tags":[],"class_list":["post-205584","post","type-post","status-publish","format-standard","hentry","category-biotech-medical","category-life-extension","category-sex"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/205584","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/662"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=205584"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/205584\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=205584"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=205584"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=205584"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}